LI-6400XT Portable Photosynthesis System

The most referenced photosynthesis system in peer-reviewed literature, the LI-6400/XT has helped researchers reveal the secrets of photosynthesis for over 20 years. We continue to provide chambers, light sources, service, and support for LI-6400/XTs.

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For the latest innovations in photosynthesis instruments, check out the LI-6800 Portable Photosynthesis System.

Chambers and Light Sources

The LI-6400XT has a variety of chambers, light sources and accessories to let you measure gas exchange, fluorescence and respiration in a variety of applications.

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Light Sources

6400-40 Leaf Chamber Fluorometer

The 6400-40 Leaf Chamber Fluorometer enables the simultaneous measurement of chlorophyll fluorescence and gas exchange over the same leaf area. The 6400-40 is designed with a uniform, integrated LED light source and PAM fluorometer that easily attaches to the LI-6400XT sensor head, giving the user complete control of the environment surrounding the 2 cm² leaf area. Measurements of combined gas exchange and fluorescence expand the LI-6400XT's usefulness in assessing a plant's response to stress.

6400-02B LED Light Source

The 6400-02B LED source is designed with red and blue LEDs (665 and 470 nm, respectively) to provide uniform intensities up to full sunlight (~2,000 µmol m^-2 s^-1) over an area of 6 cm². Mounting simply requires replacement of the upper half of the standard leaf chamber. Powered from the LI-6400XT console, the 02B allows the light intensity to be automatically controlled, which is especially critical for response curve measurements.

6400-18A RGB Light Source

Designed to be used with the Whole Plant Arabidopsis Chamber (6400-17) and Conifer Chamber (6400-22), the RGB Light Source provides a powerful tool for whole plant light response studies. The 6400-18A features LEDs with independent control of the intensity and proportions of red, green and blue. You can also define a blended color, or emulate the LED Light Source (6400-02B).

Chambers

6400-05 Conifer Chamber

The conifer chamber is constructed of acrylic for durability and lined to minimize water absorption. Long shoots (5 cm) with needles up to 3.5 cm long can be accommodated in the chamber. The 6400-05 mounts directly to the sample cell of the system's CO₂ and H₂O IRGAs.

6400-07 Needle Chamber

The 6400-07 has a 2 x 6 cm aperture and is specially designed to accommodate long-needled conifers. The chamber has foam mounting pads that hold up to five needles in the chamber. The chamber top and bottom have replaceable clear Propafilm windows for excellent light transmittance. A quantum sensor in the chamber measures light intensity at the plane of the needles.

6400-08 Clear Chamber Bottom

The 2 x 3 cm Clear Chamber Bottom has a Propafilm^® window similar to the standard chamber top and is designed for use with any 2 x 3 cm chamber top. Illuminating the bottom of a leaf may be important for survey measurements and erect foliage.

6400-09 Soil CO₂ Flux Chamber

The 6400-09 is a carefully designed 9.55 cm diameter chamber for measuring soil CO₂ flux using the LI-6400XT. A pressure equilibration tube, perforated manifold, and automated cycling regime ensure accurate and repeatable measurements. For more than two decades, LI-COR has been perfecting the science and overcoming the challenges of accurately measuring soil CO₂ flux using chamber methods.

6400-11 Narrow Leaf Chamber

The 6400-11 has a 2 x 6 cm aperture and is ideal for foliage with narrow-bladed leaves. The chamber top has a Propafilm^® window and a PAR sensor for light measurement at the leaf plane, while the chamber bottom is equipped with a thermocouple for measuring leaf temperature.

6400-15 Extended Reach 1 cm Chamber

The 6400-15 Extended Reach 1 cm Chamber is designed with a 1.0 cm diameter aperture positioned 8.5 cm away from the main body of the IRGA. Both the top and bottom chambers have Propafilm^® windows and leaf temperature is computed using energy balance. The chamber is useful for measuring small leaves that are tightly bunched in rosettes and difficult to clamp in conventional chambers.

6400-17 Whole Plant Arabidopsis Chamber

The 6400-17 features a 7 cm diameter that allows for measurements of entire Arabidopsis rosettes. The chamber has an o-ring sealed top plate and interchangeable bottom plates that allow the use of 2.5 in. (65 mm) pots or 1.5 in. (38 mm) Cone-tainers™. The 6400-17 is easily integrated with the LI-6400/6400XT sensor head by mounting to the lower leaf-chamber manifold. Measuring whole rosettes allows for repeatable measurements for growth studies that were previously difficult to achieve without the use of custom chambers. Can be used with the 6400-18A RGB light source for a lighted chamber solution.

6400-22 Opaque Conifer Chamber

The 6400-22 is constructed of an opaque material that has excellent resistance to water sorption. 7.5 cm long shoots with needles up to 3.5 cm long can be accommodated in the chamber, which mounts directly to the sample cell of the system's CO₂ and H₂O IRGAs.

6400-24 Bryophyte Chamber

The 6400-24 Bryophyte Chamber allows for loose material to be held in a shallow well for gas exchange measurements. By using the 6400-18A RGB Light Source with the chamber, measurements can be made that explore both response to light intensity and quality.

6400-89 Insect Respiration Chamber

The 6400-89 kit contains a capped cylinder (3 cm dia., 11.25 cm len.) into which the sample(s) is placed, as well as a Sample Cell Outlet Manifold Kit (p/n 9964-053) that isolates the LI-6400XT sample cell from the leaf chamber so that the instrument can be used as a stand-alone gas analyzer. The 6400-89 Insect Respiration Kit can be used to measure the respiration rate of insects, small fruits, or other respiring organisms.

Chamber Packages

Chambers and corresponding light sources are available individuals or as packages. If you are interested in both a chamber and light course, choose one of the following packages.

6400-17L: Whole Plant Arabidopsis Chamber with RGB Light Source
6400-22L: Opaque Conifer Chamber with RGB Light Source
6400-24L: Bryophyte Chamber and RGB Light Source

Accessories

6400-70 AC Module

The 6400-70 AC Module allows the LI-6400XT to be powered from mains power. It consists of a transformer and a separate battery-shaped unit that fits in the LI-6400XT console. When used with a battery, the 6400-70 will trickle charge the battery, which also provides for continued operation in the event of a mains power failure.

9964-098 Leg Extension Set

Extend the height of the LI-6400XT standard console legs an additional 12 in. Simply hand-tighten the extensions onto the standard console leg, and attach to the console as normal.

9964-053 Sample Cell Outlet Manifold

The manifold allows users to isolate the LI-6400XT sample gas analyzer so that it can be used as a stand-alone gas analyzer. The 9964-053 replaces the standard LI-6400XT bottom chamber.

6400-88 Expanded Temperature Control Kit

The 6400-88 Expanded Temperature Control Kit is an accessory for the LI-6400XT Portable Photosynthesis System that is used to increase the range of temperature control of the system sensor head block.

Dark Adapting Clips

An accessory for the 6400-40 Leaf Chamber Fluorometer that allows you to dark-adapt leaf samples is now available. Each Dark Adapting Clip consists of two "shutters", and a clip that secures the shutters over the leaf. The Dark Adapting Clip (with the shutters in the closed position) is attached to the leaf; after the leaf is fully dark-adapted, the clip is secured to the Leaf Chamber Fluorometer, the shutters are opened, and the measurement is taken. A kit including 10 clips and 10 sets of shutters is available (part number 9964-091).

6400-37 Thick Gasket Kit

Designed for use with thick, fleshy or cylindrical leaves, or leaves with thick vasculature. Thick gaskets are installed on the lower chamber to better conform around irregularly shaped leaves. An increase in distance between the lower and upper chamber halves allows the hinge to clamp evenly, reducing the risk of damage to the leaves.

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