Quantitative Western blotting is an essential tool for every step in your RNAi studies. With quantitative Western blots, you can:
The In-Cell Western Assay can be used in a functional siRNA screen measuring the effects of knockdowns in cultured cells.
In-Cell Western assays offer additional throughput for more complex studies, as well as exceptionally consistent data (Z'-factor).1 The ICW has been demonstrated as a powerful cellular assay for genome-wide RNAi screens.2
Hoffmann et al.2 used In-Cell Western screening to assess the effects of knockdowns on mTORC1-dependent phosphorylation of ribosomal protein S6 (rpS6).
In-Cell Western RNAi screening was found to be faster and less expensive than high content microscopy (IF), and gave similar or better statistical replicability.