Tissue section imaging and microscopy are effective for detailed identification of targeting agent locations within an organ. With appropriate microscopy equipment, you can assess near-infrared fluorescent-labeled targeting agent location (e.g. membrane bound, intracellular, or interstitial).
If you are developing an optical probe or targeted therapeutic for clinical use, use the Odyssey® CLx Imager to measure uptake and localization in tissues and organs ex vivo. The sections can be labeled with standard immunohistochemical protocols or in vivo as part of an animal imaging study.
Tissue sections are stained with IRDye® dye-labeled antibodies or optical probes, and imaged at 21 μM resolution for macro-level analysis. Many sections can be imaged in a single scan for screening, so that valuable microscope time is used more efficiently.
See the microscopy application page for further information on using LI-COR dyes used for microscopic applications.
Tissue section imaging plays an important role in the development of targeted therapeutics by allowing you analyze the uptake and localization of a potential therapeutic agent in tissue and organs.
Consider DigiWest® Protein Profiling Services to get a head start in the development of targeted therapeutics. Screen over 1,000 prevalidated antibodies against 50+ predefined signaling pathways to identify potential therapeutic targets or characterize functional effects.
See some published examples of quantitative Western blotsView publications