Tissue Section Imaging

Tissue section imaging and microscopy are effective for detailed identification of targeting agent locations within an organ. With appropriate microscopy equipment, you can assess near-infrared fluorescent-labeled targeting agent location (e.g. membrane bound, intracellular, or interstitial).

If you are developing an optical probe or targeted therapeutic for clinical use, use the Odyssey® CLx Imager to measure uptake and localization in tissues and organs ex vivo. The sections can be labeled with standard immunohistochemical protocols or in vivo as part of an animal imaging study.

Tissue sections are stained with IRDye® dye-labeled antibodies or optical probes, and imaged at 21 μM resolution for macro-level analysis. Many sections can be imaged in a single scan for screening, so that valuable microscope time is used more efficiently.

See the microscopy application page for further information on using LI-COR dyes used for microscopic applications.

Key Advantages of Using Odyssey Imagers

  • Quickly screen multiple sections prior to microscopic analysis
  • Permits multiplexed detection of two different targets
  • Allows quantification of staining
  • Does not require restaining after in vivo imaging experiments with IRDye optical probes
  • Reduced tissue auto-fluorescence in the near-infrared fluorescence range

Targeted Therapeutics Development

Tissue section imaging plays an important role in the development of targeted therapeutics by allowing you analyze the uptake and localization of a potential therapeutic agent in tissue and organs.

Consider DigiWest® Protein Profiling Services to get a head start in the development of targeted therapeutics. Screen over 1,000 prevalidated antibodies against 50+ predefined signaling pathways to identify potential therapeutic targets or characterize functional effects.

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Figure 1. Sagittal sections of mouse brain, immunostained for cannabinoid (CB1) and dopamine (D2) receptors to monitor changes in G protein-coupled receptor levels. The images are pseudo-colored overlays of the two staining patterns, with CB1 in red and D2 in green (dual-expression in yellow). Scale bar = 1 mm. Reprinted with permission from Kearn, CS.1
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Figure 2. Image courtesy of Dr Christopher Kearn, University of Washington.
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Figure 3. Image courtesy of Dr Christopher Kearn, University of Washington.
figure 4
Figure 4. Image courtesy of Dr Christopher Kearn, University of Washington.

References

  1. Kearn, CS. Immunofluorescent mapping of cannibinoid CB1 and dopamine D2 receptors in the mouse brain. LI-COR Biosciences application note (2004).

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