Pearl Trilogy

Pearl® Trilogy
Small Animal Imaging System

Track your probe in action with a sensitive dual-mode optical detection platform.

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Pearl Trilogy

See a Clearer Picture with Near-Infrared Fluorescence

Minimize tissue autofluorescence with high signal-to-noise ratios. Exceptional sensitivity from near-infrared (NIR) fluorescence detection provides sharp in vivo detection of optical probes and molecular targets.

Optical Imaging in a New Light

Capture an accurate representation of your optical probe and gather consistent data, image after image. In the NIR spectrum (680 nm to 820 nm), autofluorescence from tissue components is significantly reduced. As a result, animal tissue is rendered virtually transparent to excitation light in these wavelengths, allowing better penetration to detect deeper targets.

NIR fluorescence provides exceptional sensitivity and high signal-to-noise ratios for all of your measurements. Higher signal-to-noise ratios help detect, track, and validate in vivo activity with accuracy and confidence, allowing for accurate representation of the probe and its activity throughout the entire body of the animal.

figure
Don’t let visible light sources limit your detection capabilities. Capture images in the NIR window for optimal sensitivity.
Image A

A. White Light

Image B

B. Visible

Image C

C. Near-Infrared

Image D

D. Near-Infrared

Tissue autofluorescence is much lower at NIR wavelengths. Tissue autofluorescence is much higher in white light (A) and visible light (B) than in NIR wavelengths (C and D, 700nm and 800nm). Autofluorescence at 532 nm was very high.
Pearl Trilogy animation

Minimize Variability in Data Capture

Pearl Trilogy imagers are engineered with data integrity in mind. Capture coherent data that accurately reflect the clearance and biodistribution profile of your optical probe.

The FieldBrite™ Xi2 system in Pearl imagers couples NIR lasers with filter technology for a stable source of precise and powerful illumination. This leads to sharp excitation light within narrow wavelengths, causing minimal diffusion across the imaging field (CVs < 3%).

White light and LED based illumination sources diffuse excitation light across the imaging field, causing variation in signal intensities and inconsistent data. Even with appropriate excitation and emission filters, some leakage of excitation light occurs, leading to high background.

light sources
Get the sensitivity and image quality you need to detect subtle changes. NIR lasers deliver powerful, precisely-tuned excitation light to generate strong signals and provide superior image quality allowing for detection of deeper targets and low tissue autofluorescence.

Have Complete Confidence in Your Results

The Pearl Trilogy imaging system enables you to simultaneously capture the full range of signal intensity without saturation, across samples and time points.

Full Range of Signal Intensity

Don’t be limited by an imager with poor dynamic range. Imagers with limited dynamic ranges are unable to simultaneously detect signals differing more than 3 or 4 logs (1000 to 10,000X). As a result, images are saturated in areas of high signal intensity, and data are no longer coherent. Correction usually involves manual adjustment of camera exposure before every image capture.

The FieldBrite Xi2 technology in Pearl Trilogy imagers offers industry-leading 6 logs of dynamic range, leading to consistently robust, reproducible data and accurate probe characterization. Clearly capture variations in signal corresponding to the clearance and biodistribution of your optical probes over the entire course of probe clearance without adjusting any settings.

Signals in tissue samples
Imaging of both strong and weak signals in excised tissue samples. Excised organs may be imaged to examine the distribution and clearance of an optical agent. Imaging of low-signal targets such as excised lymph nodes alongside high-signal organs such as kidneys require a very wide dynamic range.

Make Every Shot Your Best Shot

Equip your in vivo imaging needs with an adaptive system that streamlines your workflow and delivers consistent, highly-reproducible data.

Best Shot, Everytime

Experiments often require moving animals in and out of the imaging area. Don’t get sidetracked re-aligning camera settings for each capture. The optical system in Pearl Trilogy imagers is designed to provide the optimal imaging setup for every data capture. Whether an experiment requires imaging one mouse, or twenty, camera and exposure remain consistent for each and every capture without readjustment.

"Ease of use is definitely a very positive attribute. The fact that you can walk up to it and get in and get out is a real advantage…"

Dr. H Charles Manning, Vanderbilt University
figure
Imaging of multiple targets in the same animal. IRDye® 680 BoneTag™ agent reveals skeletal features (700 nm, grayscale). IRDye 800CW EGF (800 nm, pseudocolor) was used to image an A431 tumor on the right hip. Overlay shows combined 700nm and 800nm images.
Pearl Trilogy

See the Pearl Trilogy in Action

Get reproducible data to assess in vivo specificity, biodistribution, and clearance of optical imaging agents and bring the sensitivity of NIR detection and high-precision optics to your small animal imaging needs.

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