Visible Fluorescent Protein Labeling Kits to Label Your Compound

Visible Fluorescent Protein Labeling Kits


Visible fluorescence protein labeling kits can be used to label antibodies and other proteins for applications such as flow cytometry, microscopy, immunohistochemistry, and other applications where fluorophore-conjugated antibodies are required.

The visible dyes included in these kits are activated with N-hydroxysuccinimide (NHS) esters, which is the most commonly used reactive group for labeling proteins. NHS esters react with primary amines, forming stable, covalent bonds.

VRDye labeling kits are based on a simple conjugation protocol that uses fast, spin column cleanup with resulting purity equivalent to purification by dialysis. Raise the pH of the preservative-free protein solution with the provided buffer, combine it with the appropriate amount of water-soluble amine-reactive dye, and separate the labeled conjugate from the free dye using the included spin columns. Labeling and purification are complete in approximately 2 hours.

Properties of Visible Fluorescence NHS Ester Dyes

Dye Absmax Emmax ε (M-1cm-1) MW (g/mole) CF*
VRDye 549 550 575 150,000 1,040 0.07
VRDye 490 491 515 73,000 1,011 0.11

*CF is a correction factor for the absorbance of the dye at Absmax to the absorbance of the protein at 280 nm.