Western Blot Membranes and Membrane Kits

Products

A low-background membrane is essential for infrared Western blot success. Background can be attributed to membrane autofluorescence or to detection of antibody non-specifically binding to the membrane.

Rely on Quality and Performance with LI-COR Western Blot Membranes

LI-COR has evaluated many different membranes for Western blotting; examples of membrane performance can be seen in the figure below. There is typically more variability in PVDF membrane performance than for nitrocellulose membranes.

LI-COR offers high-quality, prescreened Immobilon®-FL PVDF, without lot-to-lot variability, packaged in affordable kits with the Intercept Blocking Buffer (PBS or TBS), or with 4X Protein Sample Loading Buffer. LI-COR also offers nitrocellulose membranes in both sheets and rolls.

Membranes and Membrane Kit Components

Product P/N What’s Included
Odyssey Nitrocellulose Membranes 926-31090 0.22 µm; ten 7 cm × 8.5 cm sheets
Odyssey Nitrocellulose Membranes 926-31092 0.22 µm; 30 cm × 3 m roll
PBS Blocking Buffer (500 mL) and PVDF Kit 926-32098 0.45 µm; 26.5 cm × 3.75 m roll + 500 mL Intercept (PBS) Blocking Buffer
TBS Blocking Buffer (500 mL) and PVDF Kit 926-32099 0.45 µm; 26.5 cm × 3.75 m roll + 500 mL Intercept (TBS) Blocking Buffer
4X Protein Sample Loading Buffer and PVDF Membrane Kit 926-31097 0.45 µm; 26.5 cm × 3.75 m roll + 15 mL 4X Protein Sample Loading Buffer
PBS Blocking Buffer (10 x 500 mL) and PVDF Kit 829-32080 0.45 µm; 26.5 cm × 3.75 m roll + 10 × 500 mL Intercept (PBS) Blocking Buffer
TBS Blocking Buffer (10 x 500 mL) and PVDF Kit 926-32100 0.45 µm; 26.5 cm × 3.75 m roll + 10 × 500 mL Intercept (TBS) Blocking Buffer

Western Blot Membrane Selection Can Affect Your Results

Membrane Data
Western blot detection of transferrin using various vendors and brands of PVDF membrane. PVDF membranes were scanned on the Odyssey Classic Infrared Imaging System in both 700 and 800 nm channels. LI-COR offers high-quality, prescreened Immobilon-FL PVDF membranes in convenient, affordable kits.
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