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PBS Blocking Buffer and PVDF Kits
TBS Blocking Buffer and PVDF Kits
Odyssey® Nitrocellulose Membranes
4X Protein Sample Loading Buffer and PVDF Membrane Kit
A low-background membrane is essential for infrared Western blot success. Background can be attributed to membrane autofluorescence or to detection of antibody non-specifically binding to the membrane.
LI-COR has evaluated many different membranes for Western blotting; examples of membrane performance can be seen in the figure below. There is typically more variability in PVDF membrane performance than for nitrocellulose membranes.
LI-COR offers high-quality, prescreened Immobilon®-FL PVDF, without lot-to-lot variability, packaged in affordable kits with the Intercept™ Blocking Buffer (PBS or TBS), or with 4X Protein Sample Loading Buffer. LI-COR also offers nitrocellulose membranes in both sheets and rolls.
|Odyssey Nitrocellulose Membranes||926-31090||0.22 µm; ten 7 cm × 8.5 cm sheets|
|Odyssey Nitrocellulose Membranes||926-31092||0.22 µm; 30 cm × 3 m roll|
|PBS Blocking Buffer (500 mL) and PVDF Kit||926-32098||0.45 µm; 26.5 cm × 3.75 m roll + 500 mL Intercept (PBS) Blocking Buffer|
|TBS Blocking Buffer (500 mL) and PVDF Kit||926-32099||0.45 µm; 26.5 cm × 3.75 m roll + 500 mL Intercept (TBS) Blocking Buffer|
|4X Protein Sample Loading Buffer and PVDF Membrane Kit||926-31097||0.45 µm; 26.5 cm × 3.75 m roll + 15 mL 4X Protein Sample Loading Buffer|
|PBS Blocking Buffer (10 x 500 mL) and PVDF Kit||829-32080||0.45 µm; 26.5 cm × 3.75 m roll + 10 × 500 mL Intercept (PBS) Blocking Buffer|
|TBS Blocking Buffer (10 x 500 mL) and PVDF Kit||926-32100||0.45 µm; 26.5 cm × 3.75 m roll + 10 × 500 mL Intercept (TBS) Blocking Buffer|