Detection Label Reference

Detection Label Reference

The detection labels listed in this table are examples. If you do not see the detection label you want to use, check its spectral characteristics to make sure it is compatible.

Membrane

Channel	Labels	Excitation	Emission	Combined Selections Allowed
800	IRDye® 800CW, DyLight 800	785 nm	816 - 840 nm	700, 520, 488, RGB Epi
700	IRDye 680LT, IRDye 680RD, Revert 700 Total Protein Stain, Alexa Fluor 680	685 nm	721 - 740 nm	800, 520, 488, RGB Epi
520	Revert 520 Total Protein Stain, VRDye 549, Cy3, Alexa Fluor 546	520 nm	570 - 610 nm	800, 700, 488, RGB Epi
488	VRDye 490, FITC, Alexa Fluor 488	488 nm	519 - 543 nm	800, 700, 520, RGB Epi
RGB Epi	Colorimetric Protein MW Markers	NA	NA	800, 700, 520, 488, Chemi, or 630 Epi, 525 Epi
630 Epi	Pierce Reversible Protein Stain, TMB (blotting)	631 - 659 nm	Clear Window	RGB Epi
525 Epi	Ponceau S, NBT/BCIP	500 - 530 nm	Clear Window	RGB Epi
Chemi	HRP and AP chemiluminescent substrates	NA	NA	700, 520, RGB Epi

Gel (Protein and Nucleic Acid Gel)

Channel	Labels	Excitation	Emission
800	IRDye® 800CW, DyLight 800	785 nm	816 - 840 nm
700	IRDye 680LT, IRDye 680RD, SYTO 60, Coomassie Stain	685 nm	721 - 740 nm
520	GelRed, Ethidium Bromide, Midori Green, Pro-Q Diamond Phosphoprotein Gel Stain	520 nm	570 - 610 nm
488	SYBR Safe, SYBR Green, GelGreen, GreenView, Pro-Q Emerald 488 Glycoprotein Gel Stain	488 nm	519 - 543 nm
488A	SYPRO Ruby, SYPRO Orange, SYBR Gold	488 nm	570 - 610 nm
RGB Trans	Colorimetric Protein MW Markers	NA	NA
630 Trans	Coomassie Stain	631 - 659 nm	Clear Window
470 Trans	Silver Stain	447 - 472 nm	Clear Window

Multiwell Plate Assays

In-Cell Western

Channel	Labels	Excitation	Emission	Combined Selections Allowed
800	IRDye® 800CW, DyLight 800	785 nm	816 - 840 nm	700, 520, 488
700	IRDye 680RD, CellTag™ 700 Stain, Sapphire700™ Stain, DRAQ5	685 nm	721 - 740 nm	800, 520, 488
520	VRDye 549, Cy3, Alexa Fluor 546, CellTag 520 Stain	520 nm	570 - 610 nm	800, 700, 488
488	VRDye 490, FITC, Alexa Fluor 488	488 nm	519 - 543 nm	800, 700, 520

Cell Analysis

Channel	Labels	Excitation	Emission	Combined Selections Allowed
800	IRDye® 800CW, DyLight 800	785 nm	816 - 840 nm	700, 520, 488
700	IRDye 680RD, CellTag™ 700 Stain, Sapphire700™ Stain, DRAQ5, SYTO 60	685 nm	721 - 740 nm	800, 520, 488
520	VRDye 549, Cy3, Alexa Fluor 546, Red fluorescent protein (RFP), Propidium Iodide	520 nm	570 - 610 nm	800, 700, 488
488	VRDye 490, Green Fluorescent Protein (GFP), NucSpot 470, SYTOX Green	488 nm	519 - 543 nm	800, 700, 520

Absorbance Assay

Channel	Labels	Excitation	Emission	Combined Selections Allowed
630 Trans	TMB - non stopped	631 - 659 nm	Clear Window	None
470 Trans	OPD, TMB - Stopped	447 - 472 nm	Clear Window	None

Microscope Slide

Channel	Labels	Excitation	Emission	Combined Selections Allowed
800	IRDye® 800CW, DyLight 800	785 nm	816 - 840 nm	700, 520, 488, RGB Trans
700	IRDye 680RD, IRDye 680LT, Alexa Fluor 680	685 nm	721 - 740 nm	800, 520, 488, RGB Trans
520	VRDye 549, Cy3, Alexa Fluor 546, Propidium Iodide	520 nm	570 - 610 nm	800, 700, 488, RGB Trans
488	VRDye 490, FITC, Alexa Fluor 488, NucSpot 470, SYTOX Green	488 nm	519 - 543 nm	800, 700, 520, RGB Trans
RGB Trans	H&E, Alcian Blue	NA	NA	800, 700, 520, 488

Custom

Channel	Example Detection Labels	Excitation	Emission	Combined Selections Allowed
800	IRDye 800CW, DyLight 800	785 nm	816 - 840 nm	700, 520, 488, RGB Epi, RGB Trans
700	IRDye 680LT, IRDye 680RD, IRDye 700, Revert 700 Total Protein Stain, Alexa Fluor 680, CellTag 700 Stain, DRAQ5	685 nm	721 - 740 nm	800, 520, 488, RGB Epi, RGB Trans
520	Revert 520 Total Protein Stain, CellTag 520 Stain, VRDye™ 549, Cy3, Alexa Fluor 546, Red fluorescent protein (RFP)	520 nm	570 - 610 nm	800, 700, 488, RGB Epi, RGB Trans
520A	DRAQ5	520 nm	721 - 740 nm	800, 700, 488, RGB Trans
520B	Custom	520 nm	816 - 840 nm	800, 700, 488, RGB Trans
488	VRDye 490, FITC, Green Fluorescent Protein (GFP)	488 nm	519 - 543 nm	800, 700, 520, RGB Epi, RGB Trans
488A	SYPRO Ruby, SYPRO Orange	488 nm	570 - 610 nm	800, 700, 520, RGB Epi
488B	StarBright Blue 700	488 nm	721 - 740 nm	800, 700, 520, RGB Epi
488C	Custom	488 nm	816 - 840 nm	800, 700, 520, RGB Epi
Chemi	HRP and AP chemiluminescent substrates	NA	NA	700, 520, RGB Epi
RGB Epi	Colorimetric Protein MW Markers, Ponceau S, Coomassie blue stain	NA	NA	800, 700, 520, 488, Chemi, or 630 Epi, 525 Epi, 470 Epi
630 Epi	Pierce Reversible Protein Stain, TMB (blotting)	631 - 659 nm	Clear Window	RGB Epi
525 Epi	Ponceau S, NBT/BCIP	500 - 530 nm	Clear Window	RGB Epi
470 Epi	Custom	447 - 472 nm	Clear Window	RGB Epi
RGB Trans	H&E Stain, Alcian Blue	NA	NA	800, 700, 520, 520A, 488
630 Trans	Coomassie blue stain, TMB ELISA (non- stopped)	631 - 659 nm	Clear Window	None
525 Trans	Custom	500 - 530 nm	Clear Window	None
470 Trans	Silver stain, TMB – stopped ELISA, OPD	447 - 472 nm	Clear Window	None

Odyssey CLx and Odyssey DLx

Membrane

Channel	Labels	Excitation	Emission	Combined Selections Allowed
800	IRDye® 800CW, DyLight 800	785 nm	812 - 832 nm	700
700	IRDye 680LT, IRDye 680RD, Revert 700 Total Protein Stain, Alexa Fluor 680	685 nm	710 - 730 nm	800

Gel

Channel	Labels	Excitation	Emission
800	IRDye® 800CW, DyLight 800
700	IRDye 680LT, IRDye 680RD, Coomassie blue stain, SYTO 60 Stain	685 nm	710 - 730 nm

In-Cell Western

Channel	Labels	Excitation	Emission	Combined Selections Allowed
800	IRDye® 800CW, DyLight 800	785 nm	812 - 832 nm	700
700	IRDye 680RD, CellTag™ 700 Stain, Sapphire700™ Stain, DRAQ5	685 nm	710 - 730 nm	800

Cell Analysis

Channel	Labels	Excitation	Emission	Combined Selections Allowed
800	IRDye® 800CW, DyLight 800	785 nm	812 - 832 nm	700
700	IRDye 680RD, CellTag™ 700 Stain, Sapphire700™ Stain, DRAQ5	685 nm	710 - 730 nm	800

Microscope Slide

Channel	Labels	Excitation	Emission	Combined Selections Allowed
800	IRDye® 800CW, DyLight 800	785 nm	812 - 832 nm	700
700	IRDye 680RD, IRDye 680LT	685 nm	710 - 730 nm	800

Custom

Channel	Labels	Excitation	Emission	Combined Selections Allowed
800	IRDye® 800CW, DyLight 800	785 nm	812 - 832 nm	700
700	IRDye 680RD, IRDye 680LT, Alexa Fluor 680	685 nm	710 - 730 nm	800

Odyssey Fc and Odyssey XF

Membrane

Channel	Labels	Excitation	Emission	Combined Selections Allowed
800	IRDye® 800CW, DyLight 800	785 nm	816 - 846 nm	600, 700, Chemi
700	IRDye 680LT, IRDye 680RD, Revert 700 Total Protein Stain, Alexa Fluor 680	685 nm	716 - 746 nm	600, 800, Chemi
Chemi	HRP and AP chemiluminescent substrate	NA	NA	600, 700, 800
600	Custom	520 nm	573 - 637 nm	700, 800, Chemi

Gel

Channel	Labels	Excitation	Emission	Combined Selections Allowed
800	IRDye® 800CW, DyLight 800	785 nm	816 - 846 nm	600, 700
700	Coomassie Stain, SYTO 60 Stain, IRDye 680RD, IRDye 680LT	685 nm	716 - 746 nm	600
600	Ethidium Bromide, SYBR Safe	520 nm	573 - 637 nm	700