Detection Label Reference

The detection labels listed in this table are examples. If you do not see the detection label you want to use, check its spectral characteristics to make sure it is compatible.

Odyssey M

Membrane

Channel	Labels	Excitation	Emission	Combined Selections Allowed
800	IRDye® 800CW, DyLight 800	785 nm	816 - 840 nm	700, 520, 488, RGB Epi
700	IRDye 680LT, IRDye 680RD, Revert 700 Total Protein Stain, Alexa Fluor 680	685 nm	721 - 740 nm	800, 520, 488, RGB Epi
520	Revert 520 Total Protein Stain, VRDye 549, Cy3, Alexa Fluor 546	520 nm	570 - 610 nm	800, 700, 488, RGB Epi
488	VRDye 490, FITC, Alexa Fluor 488	488 nm	519 - 543 nm	800, 700, 520, RGB Epi
RGB Epi	Colorimetric Protein MW Markers	NA	NA	800, 700, 520, 488, Chemi, or 630 Epi, 525 Epi
630 Epi	Pierce Reversible Protein Stain, TMB (blotting)	631 - 659 nm	Clear Window	RGB Epi
525 Epi	Ponceau S, NBT/BCIP	500 - 530 nm	Clear Window	RGB Epi
Chemi	HRP and AP chemiluminescent substrates	NA	NA	700, 520, RGB Epi

Gel (Protein and Nucleic Acid Gel)

Channel	Labels	Excitation	Emission
800	IRDye® 800CW, DyLight 800	785 nm	816 - 840 nm
700	IRDye 680LT, IRDye 680RD, SYTO 60, Coomassie Stain	685 nm	721 - 740 nm
520	GelRed, Ethidium Bromide, Midori Green, Pro-Q Diamond Phosphoprotein Gel Stain	520 nm	570 - 610 nm
488	SYBR Safe, SYBR Green, GelGreen, GreenView, Pro-Q Emerald 488 Glycoprotein Gel Stain	488 nm	519 - 543 nm
488A	SYPRO Ruby, SYPRO Orange, SYBR Gold	488 nm	570 - 610 nm
RGB Trans	Colorimetric Protein MW Markers	NA	NA
630 Trans	Coomassie Stain	631 - 659 nm	Clear Window
470 Trans	Silver Stain	447 - 472 nm	Clear Window

Multiwell Plate Assays

In-Cell Western

Channel	Labels	Excitation	Emission
800	IRDye® 800CW, DyLight 800	785 nm	816 - 840 nm
700	IRDye 680RD, CellTag™ 700 Stain, Sapphire700™ Stain, DRAQ5	685 nm	721 - 740 nm
520	VRDye 549, Cy3, Alexa Fluor 546, CellTag 520 Stain	520 nm	570 - 610 nm
488	VRDye 490, FITC, Alexa Fluor 488	488 nm	519 - 543 nm
Chemi	HRP and AP substrates, luminescent assays	NA	NA

Any combination of up to four of the channels in the table can be selected per plate per acquisition. Different channels can be selected for different plates.

Cell Analysis

Channel	Labels	Excitation	Emission
800	IRDye® 800CW, DyLight 800	785 nm	816 - 840 nm
700	IRDye 680RD, CellTag™ 700 Stain, Sapphire700™ Stain, DRAQ5, SYTO 60	685 nm	721 - 740 nm
520	VRDye 549, Cy3, Alexa Fluor 546, Red fluorescent protein (RFP), Propidium Iodide	520 nm	570 - 610 nm
488	VRDye 490, Green Fluorescent Protein (GFP), SYTOX Green	488 nm	519 - 543 nm
Chemi	HRP and AP substrates, luminescent assays	NA	NA

Any combination of up to four of the channels in the table can be selected per plate per acquisition. Different channels can be selected for different plates.

Absorbance Assay

Channel	Labels	Excitation	Emission
630 Trans	TMB - non stopped, Bradford Protein Assay, Pierce 660nm Protein Assay, Modified Lowry Protein Assay	631 - 659 nm	Clear Window
525 Trans	OPD, BCA Protein Assay	500 - 530 nm	Clear Window
470 Trans	OPD, TMB - stopped, BCA Protein Assay	447 - 472 nm	Clear Window
RGB Trans	True color	Transmissive RGB Color

Microscope Slide

Channel	Labels	Excitation	Emission	Combined Selections Allowed
800	IRDye® 800CW, DyLight 800	785 nm	816 - 840 nm	700, 520, 488, RGB Trans
700	IRDye 680RD, IRDye 680LT, Alexa Fluor 680	685 nm	721 - 740 nm	800, 520, 488, RGB Trans
520	VRDye 549, Cy3, Alexa Fluor 546, Propidium Iodide	520 nm	570 - 610 nm	800, 700, 488, RGB Trans
488	VRDye 490, FITC, Alexa Fluor 488, NucSpot 470, SYTOX Green	488 nm	519 - 543 nm	800, 700, 520, RGB Trans
RGB Trans	H&E, Alcian Blue	NA	NA	800, 700, 520, 488

Custom

Channel	Example Detection Labels	Excitation	Emission
800	IRDye 800CW, DyLight 800	785 nm	816 - 840 nm
700	IRDye 680LT, IRDye 680RD, IRDye 700, Revert 700 Total Protein Stain, Alexa Fluor 680, CellTag 700 Stain, DRAQ5	685 nm	721 - 740 nm
700A	Custom	685 nm	816 – 840 nm
520	Revert 520 Total Protein Stain, CellTag 520 Stain, VRDye™ 549, Cy3, Alexa Fluor 546, Red fluorescent protein (RFP)	520 nm	570 - 610 nm
520A	DRAQ5	520 nm	721 - 740 nm
520B	Custom	520 nm	816 - 840 nm
488	VRDye 490, FITC, Green Fluorescent Protein (GFP)	488 nm	519 - 543 nm
488A	SYPRO Ruby, SYPRO Orange	488 nm	570 - 610 nm
488B	StarBright Blue 700	488 nm	721 - 740 nm
488C	Custom	488 nm	816 - 840 nm
Chemi	HRP and AP chemiluminescent substrates	NA	NA
RGB Epi	Colorimetric Protein MW Markers, Ponceau S	NA	NA
630 Epi	Pierce Reversible Protein Stain, TMB (blotting)	631 - 659 nm	Clear Window
525 Epi	Ponceau S, NBT/BCIP	500 - 530 nm	Clear Window
470 Epi	Custom	447 - 472 nm	Clear Window
RGB Trans	H&E Stain, Alcian Blue, Coomassie stain, Silver stain	NA	NA
630 Trans	Coomassie stain, TMB ELISA (non- stopped)	631 - 659 nm	Clear Window
525 Trans	Custom	500 - 530 nm	Clear Window
470 Trans	Silver stain, TMB – stopped ELISA, OPD	447 - 472 nm	Clear Window

Odyssey CLx and Odyssey DLx

Membrane

Channel	Labels	Excitation	Emission	Combined Selections Allowed
800	IRDye® 800CW, DyLight 800	785 nm	812 – 832 nm	700
700	IRDye 680LT, IRDye 680RD, Revert 700 Total Protein Stain, Alexa Fluor 680	685 nm	710 – 730 nm	800

Gel

Channel	Labels	Excitation	Emission
800	IRDye® 800CW, DyLight 800	785 nm	812 – 832 nm
700	IRDye 680LT, IRDye 680RD, Coomassie blue stain, SYTO 60 Stain	685 nm	710 – 730 nm

In-Cell Western

Channel	Labels	Excitation	Emission	Combined Selections Allowed
800	IRDye® 800CW, DyLight 800	785 nm	812 – 832 nm	700
700	IRDye 680RD, CellTag™ 700 Stain, Sapphire700™ Stain, DRAQ5	685 nm	710 – 730 nm	800

Cell Analysis

Channel	Labels	Excitation	Emission	Combined Selections Allowed
800	IRDye® 800CW, DyLight 800	785 nm	812 – 832 nm	700
700	IRDye 680RD, CellTag™ 700 Stain, Sapphire700™ Stain, DRAQ5	685 nm	710 – 730 nm	800

Microscope Slide

Channel	Labels	Excitation	Emission	Combined Selections Allowed
800	IRDye® 800CW, DyLight 800	785 nm	812 – 832 nm	700
700	IRDye 680RD, IRDye 680LT	685 nm	710 – 730 nm	800

Custom

Channel	Labels	Excitation	Emission	Combined Selections Allowed
800	IRDye® 800CW, DyLight 800	785 nm	812 – 832 nm	700
700	IRDye 680RD, IRDye 680LT, Alexa Fluor 680	685 nm	710 – 730 nm	800

Odyssey Fc and Odyssey XF

Membrane

Channel	Labels	Excitation	Emission	Combined Selections Allowed
800	IRDye® 800CW, DyLight 800	785 nm	816 - 846 nm	600, 700, Chemi
700	IRDye 680LT, IRDye 680RD, Revert 700 Total Protein Stain, Alexa Fluor 680	685 nm	716 - 746 nm	600, 800, Chemi
Chemi	HRP and AP chemiluminescent substrate	NA	NA	600, 700, 800
600	Custom	520 nm	573 - 637 nm	700, 800, Chemi

Gel

Channel	Labels	Excitation	Emission
800	IRDye® 800CW, DyLight 800	785 nm	816 - 846 nm
700	Coomassie Stain, SYTO 60 Stain, IRDye 680RD, IRDye 680LT	685 nm	716 - 746 nm
600	Ethidium Bromide, SYBR Safe	520 nm	573 - 637 nm

Pearl Small Animal Imaging System

Channel	Labels	Excitation	Emission
800	IRDye 800CW Optical Probes (or similar)	785 nm	816 - 846 nm
700	IRDye 680RD Optical Probes (or similar)	685 nm	716 - 746 nm
BLI	Bioluminescence Detection	N/A	450 - 650 nm
White	White Light Detection	White Light	N/A

Odyssey F Imager

Membrane

Channels^*	Labels	Excitation	Emission
800	IRDye® 800CW, DyLight 800	785 nm	813 – 832 nm
700	IRDye 680LT, IRDye 680RD, Revert 700 Total Protein Stain, Alexa Fluor 680	685 nm	710 – 730 nm
520	Revert™ 520 Total Protein Stain, VRDye™ 549 Secondary Antibodies, Cy3, Alexa Fluor 546	520 nm	570 – 610 nm
488	VRDye™ 490 Secondary Antibodies, FITC, Alexa Fluor 488	488 nm	538 – 552 nm

^*All channels shown are available in this workflow for the 4-laser Odyssey F (9180). The 700 and 800 channels are available for the 2-laser Odyssey F (9150).

Gel

Channel^*	Labels	Excitation	Emission
800	IRDye® 800CW, DyLight 800	785 nm	813 – 832 nm
700	IRDye 680LT, IRDye 680RD, Syto 60, Coomassie stain	685 nm	710 – 730 nm
520	GelRed, Ethidium Bromide, Pro-Q Diamond Phosphoprotein Gel Stain	520 nm	570 – 610 nm
488	SYBR Safe, SYBR Green, Pro-Q Emerald 488 Glycoprotein Gel Stain	488 nm	538 – 552 nm
488A	SYPRO Ruby, SYPRO Orange, SYBR Gold	488 nm	570 - 610 nm

^*All channels shown are available in this workflow for the 4-laser Odyssey F (9180). The 700 and 800 channels are available for the 2-laser Odyssey F (9150).

Microscope Slide

Channel^*	Labels	Excitation	Emission
800	IRDye® 800CW, DyLight 800	785 nm	813 – 832 nm
700	IRDye 680LT, IRDye 680RD, Alexa Fluor 680	685 nm	710 – 730 nm
520	VRDye™ 549 Secondary Antibodies, Cy3, Alexa Fluor 546, Propidium Iodide	520 nm	570 – 610 nm
488	VRDye™ 490 Secondary Antibodies, Alexa Fluor 488, NucSpot 488, STYOX Green	488 nm	538 – 552 nm

^*All channels shown are available in this workflow for the 4-laser Odyssey F (9180). The 700 and 800 channels are available for the 2-laser Odyssey F (9150).

Multiwell Plate

Channel^*	Labels	Excitation	Emission
800	IRDye® 800CW, DyLight 800	785 nm	813 – 832 nm
700	IRDye 680RD, CellTag™ 700 Stain, Sapphire700™ Stain, DRAQ 5	685 nm	710 – 730 nm
520	CellTag™ 520 Stain, VRDye™ 549 Secondary Antibodies, Cy3, Alexa Fluor 546	520 nm	570 – 610 nm
488	VRDye™ 490 Secondary Antibodies, FITC, Alexa Fluor 488	488 nm	538 – 552 nm

^*All channels shown are available in this workflow for the 4-laser Odyssey F (9180). The 700 and 800 channels are available for the 2-laser Odyssey F (9150).