|Used incorrect scan intensity setting
||Scan the gel again using a Scan Intensity of 8 in the Odyssey Application software, or use the AutoScan mode in the Image Studio™ software
|Incorrect focus offset
||Adjust the Focus Offset in the Odyssey software or Image Studio software to equal the thickness of the glass plate plus half the thickness of the gel, and scan again.
|DNA:protein complex disrupted due to heat or vortexing
||Run gel with cooled buffer. Do not vortex binding reaction.
|Not enough extract
||Add more extract to reaction.
||Minimize freeze/thaw cycles. Use protease inhibitors.
|EMSA binding reaction not fully optimized
||Use the additives in the Odyssey EMSA Buffer Kit (P/N 829-07910) to optimize the binding reaction conditions.