Near-infrared fluorescence detection (680 nm to 820 nm) is a sensitive and efficient modality for in vivo studies. NIR optical imaging provides several advantages, such as:
Light absorbance by, and background autofluorescence from animal tissue is significantly lower in the NIR region. These properties allow better light penetration and higher signal-to-noise ratios for detecting deeper targets in vivo.
In contrast to other imaging modalities, images can be captured within seconds, allowing rapid screening of mice.
Compared to other modalities, optical imaging is more economical.
These characteristics make NIR detection ideal for a variety of in vivo imaging applications.
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"The Pearl is very easy to use, and it's been very helpful for our optical imaging agent development program."
Optical probes aid in visualizing and monitoring the pharmacology of the targeting agent of interest. Near-infrared labeled targeting agents include antibodies, proteins, peptides, ligands, and drugs, among others.
Developing an optical probe for diagnostic or therapeutic applications involves several validation steps. Characterization of optical probes should be done in vitro to confirm specificity to the target before moving to animal studies, as in vivo studies can be very costly. Biological characterization of the optical probe in vivo and ex vivo can follow using the Pearl® Trilogy Small Animal Imaging System.
Target specificity studies evaluate the binding specificity and affinity of the probe to targets in vivo. These studies also help identify any non-specific interactions of the probe. Cell surface receptors and transporters are the most common targets for in vivo specificity studies.
Cell surface receptors such as EGF and integrin receptors are commonly targeted for in vivo imaging in cancer. Tumors often over-express cell surface receptors, providing easy access for targeting molecules such as ligands, antibodies, or drugs. These targeting molecules bind to the receptors, and thus can be evaluated for imaging studies.
For in vivo imaging of cancer, cell surface transporters such as glucose transporters are also commonly employed as targets. This is because tumors are often more metabolically active than surrounding normal cells, and may have an increased rate of glucose metabolism and cell surface glucose transporter activity.1, 6 For instance, PET imaging employs the binding property of FDG to GLUT transporters in imaging.
Low autofluorescence from tissue components and deeper penetration of light in the near-infrared spectrum allows sensitive detection and semi-quantitative assessment of probe binding affinity to the intended target. Non-specific interactions can also be detected with high sensitivity. Additionally, uniform laser illumination across the entire imaging area in the Pearl Trilogy enables accurate in vivo measurement of specificity.
Probes can be developed against several receptor and transporter targets. For instance, the GLUT1 glucose transporter can be targeted with a fluorescent-labeled IRDye® 2-deoxyglucose (2-DG) agent for longitudinal studies of tumor progression. 2 EGF and integrin receptors can be targeted using IRDye EGF agent 3 or IRDye 800CW RGD optical probes,4, 5 respectively.
Biodistribution and clearance assessments track the movement of a probe in order to understand its uptake, localization, distribution, and elimination within the animal.
Biodistribution studies can identify regions and time of specific and non-specific retention of the probe in organs and tissues.
In vivo clearance studies monitor the elimination of the optical probe over time, in live animals. Knowledge of probe elimination is important to optimize both imaging time and dose, as well as to identify any specific or non-specific retention, which may impact imaging results.
Deeper light penetration and low autofluorescence from animal tissues make near-infrared detection ideal for tracking biodistribution (in vivo and ex vivo) and clearance with high sensitivity. Low background yields excellent signal-to-noise ratios for monitoring movement of probes in vivo.
The Pearl Trilogy Imaging System can perform rapid time-lapse and longitudinal in vivo NIR imaging without the need to manually adjust camera settings between scans. For ex vivo analysis, the Organ Tray Base and disposable organ trays provide a non-heated, flat surface for imaging on the Pearl Trilogy system. IRDye infrared dyes IRDye 800CW and IRDye 680RD provide excellent performance for in vivo and ex vivo biodistribution imaging.