IRDye® 800CW EGF Optical Probe

IRDye EGF Probe to Measure Overexpression of EGF Receptors.

IRDye 800CW EGF Optical Probe is a near-infrared (NIR) fluorescently-labeled recombinant human epidermal growth factor (EGF). This optical imaging agent is composed of a recombinant EGF polypeptide containing 54 amino acid residues (molecular weight = 6.2 kDa) conjugated to the LI-COR IRDye 800CW infrared dye.

IRDye 800CW EGF provides a versatile probe that can be used for in vitro and in vivo assays, as well as whole organ and tissue section analysis. In vivo assays of EGF binding have proven effective in monitoring specific solid tumor models that exhibit overexpression of EGFR.

Epidermal growth factor receptor (EGFR) is one of a family of receptor tyrosine kinases found on the surface of epithelial cells, to which EGF binds. Many types of cancer cells have abnormally high EGFR levels on the cell surface (table below).

Tumor Type % of Tumors Over-Expressing EGFR
Head and Neck 80-100
Renal Cell 50-90
Non-small-cell Lung 40-80
Glioma 40-63
Ovarian 35-70
Bladder 31-48
Pancreatic 30-50
Colon 25-77
Breast 14-91

Frequency of elevated EGFR expression in different types of epithelial tumors1.

Learn about other LI-COR optical probes.

Applications

Absorbance and Emission Spectra

Determine Binding Specificity with IRDye 800CW EGF

In-Cell Western assays on the Odyssey® CLx, Odyssey Classic, or Odyssey Sa Infrared Imaging Systems were used to determine binding specificity of the probe in vitro for various cell lines.

Two different cell lines known to over-express the EGFR were characterized by evaluating: a) binding of IRDye 800CW EGF; b) IRDye 800CW only; and c) a competition assay using unlabeled EGF to compete for EGFR sites.

In vivo Data Example

Intravenous administration of IRDye 680RD EGF (2 nmol) binds to the A431 tumor located on the right hip. Image captured 24 h post injection on the Pearl® Impulse Imaging System.

References

  1. Herbst, R.S., & Shin, DM (2002). Cancer, (94) 5 DOI 10.1002/cncr.10372.10372.
  2. Kovar, J. L., Volcheck, W. M., Chen, J., & Simpson, M. A. (2007). Anal Biochem, 361(1), 47–54. doi: 10.1016/j.ab.2006.11.021.

Selected P/N: 926-08446

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