IRDye® 800CW Protein Labeling Kits

IRDye 800CW Labeling Kits for Antibody and Protein Labeling.

IRDye 800CW Protein Labeling Kits can be used to label antibodies and other proteins for applications such as Western blots, In-Cell Western assays, in vivo imaging, and whole organ or tissue section assays.

High Molecular Weight

Optimized for labeling 1.0 mg amounts of protein with molecular weight 45 - 200 kDa.

This kit contains:

  • 3 x 0.1 mg IRDye 800CW Reactive Dye vials
  • 0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9
  • 25 mL 1X PBS
  • 0.5 mL ultra-pure water
  • 3 x Pierce® Zeba Desalting Spin Columns (P/N 89891)
  • Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.
  • Pierce Zeba Desalting Spin column instructions
  • Protocol for IRDye 800CW Protein Labeling Kit - High MW

Low Molecular Weight

Optimized for labeling 1.0 mg of protein with molecular weight 14 - 45 kDa.

This kit contains:

  • 3 x 0.1 mg IRDye 800CW Reactive Dye vials
  • 0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9
  • 25 mL 1X PBS
  • 0.5 mL ultra-pure water
  • 3 x Pierce Zeba Desalting Spin Columns (P/N 89891)
  • Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.
  • Pierce Zeba Desalting Spin column instructions
  • Protocol for IRDye 800CW Protein Labeling Kit - Low MW

Microscale

Optimized for labeling 100 μg of protein with molecular weight 14 - 200 kDa.

This kit contains:

  • 3 x 0.1 mg IRDye 800CW Reactive Dye vials
  • 0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9
  • 25 mL 1X PBS
  • 0.5 mL ultra-pure water
  • 3 x Pierce Zeba Desalting Spin Columns (P/N 89892)
  • Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.
  • Pierce Zeba Desalting Spin Column instructions
  • Protocol for IRDye 800CW Protein Labeling Kit - Microscale

Selected P/N: 928-38040,928-38042,928-38044

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