Purity and Specificity
Isolation of specific antibodies was accomplished by affinity chromatography using pooled IgG covalently linked to agarose. Based on ELISA, this antibody reacts with the heavy and light chains of goat IgG and with sheep IgG. This antibody was tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with human, mouse, rabbit, rat, chicken. guinea pig, hamster, swine, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications.
Highly recommended for:
- Western Blot
- In-Cell Western Assay
- On-Cell Western Assay
- Protein Array
- Small Animal Imaging
- 2D Gel Detection
- Tissue Section Imaging
IRDye 680RD secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
|Odyssey® Western blot detection||1:15,000||1:5,000 - 1:25,000|
|In-Cell Western assay detection||1:800||1:200 - 1:800|
Optimum dilutions will vary and should be determined empirically.