WesternSure® ECL Stripping Buffer for Chemiluminescent Westerns

WesternSure ECL Stripping Buffer for Chemiluminescent Westerns.

WesternSure ECL Stripping Buffer is ideal for stripping and reprobing chemiluminescent Western blots. This Western blot stripping buffer:

  • Removes primary and secondary antibodies from PVDF or nitrocellulose membranes
  • Maintains target antigen integrity for efficient reprobing
  • Does not require hazardous shipping, unlike many other stripping buffers

Stripping buffers are not recommended if bands need to be quantified.

Note: WesternSure ECL Stripping Buffer is not recommended for loading amounts over 30 µg. It will demonstrate decreased performance when used at 2X or 5X concentration and heating the solution will not increase the stripping efficiency.

Specifications

WesternSure ECL Stripping Buffer is provided as a 5X concentrated solution. It is stored at room temperature.

Data Example of Stripping and Reprobing with WesternSure ECL Stripping Buffer

WesternSure ECL Stripping Buffer data
WesternSure ECL Stripping Buffer is effective for reprobing with different antibodies. Original: EGF-stimulated A431 lysate (10 and 5 µg total protein) was probed with mouse anti-ERK2 followed by detection with WesternSure HRP Goat anti-Mouse (P/N 926-80010) and WesternSure PREMIUM Chemiluminescent Substrate (P/N 926-95000). The blot was exposed to film for 20 sec. Stripped: The blot was then stripped with WesternSure ECL Stripping Buffer (P/N 926-92000), followed by detection with WesternSure PREMIUM, and exposed to film for 10 min. Reprobed: The blot was re-blocked with and reprobed with β-Actin Monoclonal (P/N 926-42212) followed by detection with HRP Goat anti-Mouse and WesternSure PREMIUM. The blot was exposed to film for 20 sec.

Selected P/N: 926-92000

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