Chicken IgY, whole molecule. IgY is the original designation for the IgG-like protein found in both serum and egg yolk.
Purity and Specificity
The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis and/or ELISA, this antibody reacts with whole molecule chicken IgY, and with the light chains of other chicken immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, goat, guinea pig, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ Assay applications.
Highly recommended for:
- Western Blot
- In-Cell Western Assay
- On-Cell Western Assay
- Protein Array
- Small Animal Imaging
- 2D Gel Detection
- Tissue Section Imaging
IRDye 680RD Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
|Odyssey® Western blot detection||1:20,000||1:5,000 - 1:25,000|
|In-Cell Western Assay detection||1:800||1:200 - 1:1,200|
Optimum dilutions will vary and should be determined empirically.
- P/N 925-68075: RRID AB_2814924
- P/N 926-68075: RRID AB_10974977