Purity and Specificity
The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on ELISA, this antibody reacts with heavy and light chains of goat IgG and sheep IgG. This antibody was tested by dot blot and/or solid phase adsorbed for minimal cross-reactivity with human, mouse, rabbit, rat, chicken, guinea pig, hamster, swine, and horse serum proteins but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.
- Western blotting
VRDye 549 secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C before and after reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
|Western Blot Detection||1:10,000||1:5,000 - 1:15,000|
Suggested working dilutions are given as a guide only. Optimum dilutions will vary and should be determined empirically.