α-Tubulin primary antibody can be used as an internal loading control (ILC) for normalization.
The expression of α-tubulin, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.
Once validated, α-tubulin primary antibodies can be used for the detection of α-tubulin when performing two-color detection.
Detect α-Tubulin Mouse Monoclonal Antibody with IRDye® Goat anti-Mouse or IRDye Donkey anti-Mouse secondary antibodies.
Reactivity and Specificityα-Tubulin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.
Do not aliquot the antibody.
|Properties||α-Tubulin Mouse Monoclonal Antibody (P/N 926-42213)|
|Species Cross-Reactivity||Human, mouse, rat, monkey|
|Target Molecular Weight||52 kDa|
|Specificity/Sensitivity||Detects endogenous levels of total α-tubulin protein. α/β-tubulin heterodimers form the tubulin subunit common to all eukaryotic cells.|
|Immunogen||Full-length chicken α-tubulin (purified from brain extracts)|
|Tested Applications||Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F)|