α-Tubulin Mouse Monoclonal Antibody for Normalization

alpha-Tubulin Mouse Monoclonal Antibody

α-Tubulin primary antibody can be used for detection of α-Tubulin by Western blot or by In-Cell Western assay.

You need to validate the expression of tubulin, or any housekeeping protein (HKP), to ensure that its expression does not change under experimental conditions. Otherwise, quantitation cannot accurately be performed.

Once validated, α-tubulin primary antibodies can be used for detection of α-tubulin as a normalization antibody when performing two-color detection

Detect α-Tubulin Mouse Monoclonal Antibody with IRDye® Goat anti-Mouse or IRDye Donkey anti-Mouse secondary antibodies.

Other options for housekeeping protein normalization

Reactivity and Specificity

α-Tubulin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.

Do not aliquot the antibody.

Properties α-Tubulin Mouse Monoclonal Antibody (P/N 926-42213)
Species Cross-Reactivity Human, mouse, rat, monkey
Target Molecular Weight 52 kDa
Isotype Mouse IgG1
Specificity/Sensitivity Detects endogenous levels of total α-tubulin protein. α/β-tubulin heterodimers form the tubulin subunit common to all eukaryotic cells.

Selected P/N: 926-42213

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When using an HKP as your normalization strategy, it’s important to validate the HKP for each experiment to ensure its expression is stable.

Many factors can influence expression including tissue, treatment, and cell density.

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