IRDye 680RD NHS Ester Used to Label Primary or Secondary Amines.

IRDye® 680RD NHS Ester

IRDye 680RD NHS Ester Used to Label Primary or Secondary Amines.

IRDye infrared dyes from LI-COR Biosciences are available for researchers developing applications using near-infrared (NIR) fluorescence. These unique dyes are used in a variety of NIR imaging applications including Western blotting, plate-based assays, protein arrays, tissue section imaging, and molecular activity measurements.

Standard NHS ester chemistry is used to produce custom probes labeled with LI-COR IRDye infrared dyes. The NHS ester reactive group provides the functionality for labeling primary and secondary amines, such as lysine residues in proteins.

IRDye® 680RD NHS Ester reaction
NHS Ester reacts with a primary aliphatic amine, such as lysine.
IRDye® 680RD NHS Ester amide bond
Dye label is attached through amide bond.
Which 700 Channel Dye Should I Use?

Dye Structure


  • Chemical Formula: C43H45CIN4Na2O13S3
  • Molecular Weight: 1003.46 g/mol
  • Exact Mass: 1002.16

Absorption and Emission Spectra

Solvent Ext. Coeff. (M-1cm-1) Absorption Maxima Emission Maxima
Methanol 170,000 680 nm 694 nm
1X PBS 165,000 672 nm 694 nm
PBS: Methanol 165,000

Absorption and Emission Spectra in 1X PBS

IRDye 680RD Absorption and Emission Spectra

Selected P/N: 929-70050,929-70051,929-70052



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