IRDye 680RD BoneTag Optical Probe is a calcium-chelating compound conjugated to IRDye 680RD near-infrared dye. Using IRDye NIR dyes extends fluorescence signal detection to the near-infrared fluorescent region of the spectrum without affecting the compound’s ability to function as a marker of the mineralization process. NIR fluorescence detection improves depth of penetration due to low tissue autofluorescence, translating to low background interference.
Calcium-chelating compounds have been used effectively for the detection of bone mineralization, growth, and morphological changes, including tetracycline derivatives, xylenol orange, alizarin, calcein, and fluorescein. NIR-labeled pamidronate has also been successfully used for detection of arterial calcification in non-invasive optical imaging1.
Absorbance and Emission Spectra
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- Kovar, J. L., Xu, X., Draney, D., Cupp, A., Simpson, M. A., & Michael Olive, D. (2011). Anal Biochem, 416(2), 167–173. doi: 10.1016/j.ab.2011.05.011.