IRDye 800CW Carboxylate is a Non-Reactive Control Imaging Studies.

IRDye® 800CW Carboxylate

IRDye 800CW Carboxylate is a Non-Reactive Control Imaging Studies.

Assays that use IRDye 800CW conjugates (such as in vivo imaging and cell binding assays) may require a "dye-only" control for potential effects or retention of the dye.

Carboxylate (non-reactive) form of IRDye 800CW is an ideal control.

Note: The carboxylate dye has no reactive group and cannot be used for labeling.

IRDye® 800CW Carboxylate clearance kinetics data
Clearance kinetics of IRDye 800CW carboxylate. A single SCID mouse was injected with 1 nmol of non-reactive IRDye 800CW dye, and clearance monitored over time as indicated. Pseudocolor fluorescence is superimposed on a white light image. IRDye 800CW carboxylate dispersed rapidly and was completely cleared after 48 h. Originally published in Kovar, J., et al. (2007) Anal Biochem 367:1-12.

How is IRDye 800CW Carboxylate Used?

In vitro Cell-based Assays

As a control for cell-based assays that monitor binding of a dye-labeled agent.

  • Validation of optical agents for in vivo administration
  • Evaluation of binding specificity

In vivo Imaging

For evaluation of behavior and clearance of the dye itself.

  • Timing of dye clearance from the animal's body
  • Retention of dye in certain organs or sites (e.g., liver or kidneys)

Labeling Reaction Reference

As a standard to determine the amount of unreacted ("free") dye after IRDye 800CW conjugation and purification.

Residual unreacted dye may cause:

  • Artificially high values when dye/protein (D/P) ratio is calculated
  • Increased background fluorescence in biological assays

Dye Structure

Properties

  • Chemical Formula: C46H50N2Na4O15S4
  • Molecular Weight: 1091.10 g/mol
  • Exact Mass: 1090.17

Selected P/N: 929-08972,929-09406,929-70122,929-70123,929-70124,929-70125,929-70126

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