IRDye® 800CW BoneTag Optical Probe

IRDye 800CW BoneTag for Bone Growth and Mineralization Studies.

IRDye 800CW BoneTag Optical Probe is a calcium-chelating compound conjugated to IRDye 800CW near-infrared dye. Using IRDye NIR dyes extends fluorescence signal detection to the near-infrared fluorescent region of the spectrum without affecting the compound’s ability to function as a marker of the mineralization process. NIR fluorescence detection improves depth of penetration due to low tissue autofluorescence, translating to low background interference.

Calcium-chelating compounds have been used effectively for the detection of bone mineralization, growth, and morphological changes, including tetracycline derivatives, xylenol orange, alizarin, calcein, and fluorescein. NIR-labeled pamidronate has also been successfully used for detection of arterial calcification in non-invasive optical imaging1.

The ability to visualize bone anatomy and structures for an extended period facilitates use of the IRDye BoneTag in conjunction with a second optical agent specific for a primary target (i.e., tumor tissue). In addition, IRDye 800CW BoneTag may prove effective for studies dealing with bone metastasis and bone remodeling studies.

Learn about other LI-COR optical probes.

Applications

Absorbance and Emission Spectra

Data Example

Bone-targeted binding was examined using MC3T3 (osteoblast) cells in a cell-based assay. MC3T3 cells exhibited a dose dependent increase in signal when incubated with IRDye 800CW BoneTag. Incubation of labeled BoneTag with A431 cells (epidermoid carcinoma), a non-osteoblast cell line, showed low non-specific binding.

References

  1. Zaheer A et al. (2001) In vivo near-infrared fluorescence imaging of osteoblastic activity. Nat Biotechnol, 19. 1148-54.
  2. Kovar, J. L., Xu, X., Draney, D., Cupp, A., Simpson, M. A., & Michael Olive, D. (2011). Anal Biochem, 416(2), 167–173. doi: 10.1016/j.ab.2011.05.011.

Selected P/N: 926-09375

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