IRDye® 680LT Goat anti-Mouse IgG Secondary Antibody

IRDye 680LT Goat anti-Mouse IgG Secondary Antibody

Immunogen

Mouse IgG paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using pooled mouse IgG covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, and IgG3, and with the light chains of mouse IgM and IgA. This antibody was tested by dot blot and and/or solid-phase adsorbed for minimal cross-reactivity with human, rabbit, goat, rat, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

Applications

Recommended for:

  • Western Blot
  • Protein Array
  • Immunohistochemistry
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging

Not Recommended for:

  • Small Animal Imaging
  • In-Cell Western Assay
  • On-Cell Western Assay

Formulation

IRDye 680LT secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application Recommended Suggested Range
Odyssey Western blot detection 1:20,000 1:20,000 - 1:50,000
Other User optimized

Optimum dilutions will vary and should be determined empirically.

Example Data

IRDye® 680LT Goat anti-Mouse IgG Secondary Antibody data
Western blot detection of ERK2 in Hela lysate (5 µg – 78 ng). Samples loaded in 4X protein loading buffer (P/N 928-40004), resolved on a 10% Bis-Tris gel, and transferred to Odyssey® nitrocellulose (P/N 926-31090). The membrane was probed with mouse anti-ERK2 (Santa Cruz P/N sc-1647) followed by detection with IRDye 680LT Goat anti-Mouse IgG (P/N 926-68020).

Selected P/N: 926-68020,925-68020

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IRDye 680LT secondary antibodies are not intended for use in In-Cell Western assays or in vivo imaging applications.

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