Vinculin is a highly conserved cytoskeletal protein. The Vinculin primary antibody can be used as an internal loading control for normalization and is particularly effective when detecting high molecular weight targets.
The expression of Vinculin, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.
Once validated, Vinculin primary antibodies can be used for the detection of Vinculin when performing multiplex Western blot detection.
Detect Vinculin Rabbit Monoclonal Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.
Reactivity and Specificity
Vinculin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.
Do not aliquot the antibody.
|Properties||Vinculin Rabbit Monoclonal Antibody (P/N 926-42215)|
|Species Cross-Reactivity||Human, mouse, rat, monkey|
|Target Molecular Weight||124 kDa|
|Specificity/Sensitivity||Detects endogenous levels of total vinculin protein and metavinculin, a 145 kDa splice variant of vinculin|
|Immunogen||A recombinant protein that is specific to the amino terminus of human vinculin protein|
|Tested Application||Western blot (WB), Immunohistochemistry (IHC), Flow Cytometry (F)|