Vinculin Rabbit Monoclonal Antibody for Normalization

Vinculin Rabbit Monoclonal Antibody for Normalization

Vinculin is a highly conserved cytoskeletal protein. The Vinculin primary antibody can be used as an internal loading control for normalization and is particularly effective when detecting high molecular weight targets.

The expression of Vinculin, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.

Once validated, Vinculin primary antibodies can be used for the detection of Vinculin when performing multiplex Western blot detection.

Detect Vinculin Rabbit Monoclonal Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.

Other options for housekeeping protein normalization

Reactivity and Specificity

Vinculin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.

Do not aliquot the antibody.

Properties Vinculin Rabbit Monoclonal Antibody (P/N 926-42215)
Species Cross-Reactivity Human, mouse, rat, monkey
Target Molecular Weight 124 kDa
Isotype Rabbit IgG
Specificity/Sensitivity Detects endogenous levels of total vinculin protein and metavinculin, a 145 kDa splice variant of vinculin
Immunogen A recombinant protein that is specific to the amino terminus of human vinculin protein
Tested Application Western blot (WB), Immunohistochemistry (IHC), Flow Cytometry (F)

Vinculin Rabbit Monoclonal Antibody Detected in C32 Cells

Vinculin Rabbit Monoclonal antibody detected in C32 cells
Vinculin Rabbit Monoclonal Antibody detected in C32 cells. C32 lysates were diluted from 2.5 μg to 156 ng. Lysates were separated on 4-12% Bis-Tris gels, electrophoresed at 200V for 45 minutes in MES Running Buffer, and transferred to nitrocellulose membranes in Tris-Glycine buffer at 100V for 65 minutes. Blots were blocked in Intercept® (PBS) Blocking Buffer (P/N 927-70001), probed with Vinculin Rabbit Monoclonal Antibody, and detected on an Odyssey® CLx Imager.

Selected P/N: 926-42215

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When using an HKP as your normalization strategy, it’s important to validate the HKP for each experiment to ensure its expression is stable.

Many factors can influence expression including tissue, treatment, and cell density.

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