Purity and Specificity
The antibody was isolated by affinity chromatography using antigens coupled to agarose beads. Based on ELISA, this antibody reacts with heavy and light chains of mouse IgG and the light chains of mouse IgM and IgA. This antibody was tested by ELISA and/or solid phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, horse, human, rabbit, and sheep serum proteins but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.
- Western blotting
VRDye 549 secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C before and after reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
|Western Blot Detection||1:10,000||1:5,000 - 1:15,000|
Suggested working dilutions are given as a guide only. Optimum dilutions will vary and should be determined empirically.