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IRDye® 680RD Protein Labeling Kits

Use IRDye 680RD Labeling Kits to Label Antibodies and Proteins.

IRDye 680RD Protein Labeling Kits can be used to label antibodies and other proteins for Western blotting, In-Cell Western assays, in vivo imaging, and whole organ or tissue section applications.

High Molecular Weight

Optimized for labeling 1.0 mg of protein with molecular weight 45 - 200 kDa.

This kit contains:

  • 3 x 0.1 mg IRDye 680RD Reactive Dye vials
  • 0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9
  • 25 mL 1X PBS
  • 0.5 mL ultra-pure water
  • 3 x Pierce® Zeba Desalting Spin Columns (P/N 89891)
  • Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.
  • Pierce Zeba Desalting Spin column instructions
  • Protocol for IRDye 680RD Protein Labeling Kit - High MW

Low Molecular Weight

Optimized for labeling 1.0 mg of protein with molecular weight 14 - 45 kDa.

This kit contains:

  • 3 x 0.5 mg IRDye 680RD Reactive Dye vials
  • 0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9
  • 25 mL 1X PBS
  • 0.5 mL ultra-pure water
  • 3 x Pierce Zeba Desalting Spin Columns (P/N 89891)
  • Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.
  • Pierce Zeba Desalting Spin column instructions
  • Protocol for IRDye 680RD Protein Labeling Kit - Low MW

Microscale

Optimized for labeling 100 μg of protein with molecular weight 14 - 200 kDa.

This kit contains:

  • 3 x 0.1 mg IRDye 680RD Reactive Dye vials
  • 0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9
  • 25 mL 1X PBS
  • 0.5 mL ultra-pure water
  • 3 x Pierce Zeba Desalting Spin Columns (P/N 89892)
  • Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.
  • Pierce Zeba Desalting Spin Column instructions
  • Protocol for IRDye 680RD Protein Labeling Kit - Microscale

Selected P/N: 928-38072,928-38074,928-38076

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