GAM/GAR CellTag 520 Stain ICW Kits provide detection reagents for cell-based In-Cell Western™ Assays. This cost-effective normalization method makes quantification of the target protein more precise. CellTag 520 Stain accumulates in the nucleus and cytoplasm of permeabilized cells allowing for accurate normalization to cell number.
This kit includes reagents to perform an In-Cell Western Assay and CellTag 520 Stain to normalize well-to-well variations in cell number.
In-Cell Western Assay Overview
- IRDye® 800CW Goat anti-Mouse Secondary Antibody, 0.5 mg
- IRDye 680RD Goat anti-Rabbit Secondary Antibody, 0.5 mg
- Intercept® (TBS) Blocking Buffer, 1 x 500 mL
- CellTag 520 Stain, 2 x 50 nmol
- Kit Protocol