IRDye QC-1 is the first non-fluorescent (dark) quencher compatible with a wide range of visible and near-infrared fluorophores (~500-800 nm). It has the widest available quenching range, so you do not need to carefully match the donor's fluorescence spectrum with the acceptor's absorbance. IRDye QC-1 quenches common fluorophores with >97% efficiency1.
For more information, see “Spectral Overlap of IRDye QC-1 Absorption with Common Donor Fluorophores” below.
IRDye QC-1 NHS ester can be used for FRET (fluorescence resonance energy transfer) applications such as protease assays. Recently, it has been paired with IRDye 800CW in optoacoustic studies.IRDye Infrared Dyes Overview
- Chemical Formula: C53H62C|N4Na3O16S4
- Molecular Weight: 1243.75 g/mol
- Exact Mass: 1242.24
|Solvent||Ext. Coeff. (M-1cm-1)||Absorption Maxima|
|1X PBS||96,000||737 nm|
Absorption Spectrum in 1X PBS
Absorption Spectrum in Methanol
Spectral Overlap of IRDye QC-1 Absorption with Common Donor Fluorophores
- Peng, X et al. A nonfluorescent, broad range quencher dye for Fõrster resonance energy transfer assays. Anal Biochem. 388(2):220-28 (2009).