IRDye® 680RD Donkey anti-Guinea Pig IgG Secondary Antibody

IRDye 680RD Donkey anti-Guinea Pig IgG Secondary Antibody

Immunogen

Guinea Pig IgG, whole molecule

Purity and Specificity

Isolation of specific antibodies was accomplished by immunoaffinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis and/or ELISA, this antibody reacts with whole molecule guinea pig IgG, and with the light chains common to other guinea pig immunoglobulins. This antibody was tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, chicken, goat, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western assay applications.

Applications

Highly recommended for:

  • Western Blot
  • In-Cell Western Assay
  • On-Cell Western Assay
  • Protein Array
  • Immunohistochemistry
  • Small Animal Imaging
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging

Formulation

IRDye 680RD secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application Recommended Suggested Range
Odyssey® Western blot detection 1:15,000 1:5,000 - 1:25,000
In-Cell Western assay detection 1:800 1:200 - 1:1,200
Other User optimized

Optimum dilutions will vary and should be determined empirically.

Selected P/N: 926-68077,925-68077

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