WesternSure® Goat anti-Mouse HRP Secondary Antibody

WesternSure Goat anti-Mouse HRP Secondary Antibody.

Immunogen

Mouse IgG paraproteins

Purity and Specificity

The antibody was purified by affinity chromatography on pooled mouse IgG covalently linked to agarose. Based on ELISA and/or flow cytometry, the antibody reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, and IgG3, along with the light chains of mouse IgM and IgA. The antibody has minimal cross-reactivity with human immunoglobulins.

Application

  • Chemiluminescent Western blots

Formulation and Recommended Dilutions

The antibody is supplied as purified immunoglobulin, supplied in 50% glycerol and 50% PBS, pH 7.4. No preservative has been added. Store at 4 °C.

Substrate Recommended Dilution Range
WesternSure PREMIUM Substrate 1:5,000 – 1:100,000
Other User optimized

Selected P/N: 926-80010

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