IRDye® 800CW Donkey anti-Goat IgG Secondary Antibody

IRDye 800CW Donkey anti-Goat IgG Secondary Antibody

Immunogen

Goat IgG, whole molecule

Purity and Specificity

The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on electrophoresis, this antibody reacts with the heavy and light chains of goat IgG, and with the light chains common to most goat immunoglobulins. This antibody also reacts with sheep IgG. No antibody was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with chicken. guinea pig, Syrian hamster, horse, human, mouse, rat, and rabbit serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

Applications

Highly recommended for:

  • Western Blot
  • In-Cell Western Assay
  • On-Cell Western Assay
  • Protein Array
  • Immunohistochemistry
  • Small Animal Imaging
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging
  • Virus Titration Assay
Note: Do not use this secondary antibody in combination with any secondary antibody whose host is goat (for example, IRDye 680RD Goat anti-Rabbit) for two-color Western blot detection.

Formulation

IRDye 800CW secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application Recommended Suggested Range
Odyssey® Western blot detection 1:15,000 1:5,000 - 1:25,000
Other User optimized

Optimum dilutions will vary and should be determined empirically.

Selected P/N: 926-32214,925-32214

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