Intercept™ (TBS) Blocking Buffer

Use Cost-Effective Intercept (TBS) Blocking Buffer

Intercept (TBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for reproducible quantitative Western blots and other immunoassays. Intercept (TBS) Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS).

  • Contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies.
  • Can be used with the widest range of targets for Western blot detection.
  • Ideal for primary antibodies that are optimized for Tris-based buffer systems.
  • Can be used for detection of phosphorylated targets.
  • Has superior performance for alkaline phosphatase detection.
  • Excellent choice for biotin-streptavidin detection.

For larger pack sizes, please contact your local sales office.

Formulation

Intercept (TBS) Blocking Buffer is a ready-to-use blocker formulation in TBS. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection. It is stored at 4 °C and is also available in kits with Immobilon®-FL PVDF and nitrocellulose membranes.

Figure 1: Western blot protocol demonstrating the comparison of Intercept Blocking Buffer to Odyssey Blocking Buffer. Blots with serial dilution of mouse heart tissue extract were probed for Stat3 Mouse mAb and p38 MAPK Rabbit Ab, detected with IRDye® 800CW Goat anti-Mouse IgG (P/N 925-32210) and IRDye 680LT Goat anti-Rabbit IgG (P/N 925-68021), and scanned together on an Odyssey CLx Imaging System.

Intercept (TBS) Blocking Buffer can be used for many immunoassays and applications, including:

Selected P/N: 927-60001,927-60003,927-60010

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