Peroxidase from horseradish roots
Purity and Specificity
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with peroxidase from horseradish roots. It may cross-react with peroxidase from other sources. The conjugate has been specifically tested and qualified for Western blot applications
- Western Blot
IRDye 800CW secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
|Odyssey® Western blot detection||1:15,000||1:5,000 - 1:25,000|
Optimum dilutions will vary and should be determined empirically.