Convert to NIR Fluorescence Detection with IRDye 800CW Rabbit anti-HRP

IRDye® 800CW Rabbit anti-HRP

Convert to NIR Fluorescence Detection with IRDye 800CW Rabbit anti-HRP

Immunogen

Peroxidase from horseradish roots

Purity and Specificity

The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with peroxidase from horseradish roots. It may cross-react with peroxidase from other sources. The conjugate has been specifically tested and qualified for Western blot applications

Application

  • Western Blot

Formulation

IRDye 800CW secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application Recommended Suggested Range
Odyssey® Western blot detection 1:15,000 1:5,000 - 1:25,000
Other User optimized

Optimum dilutions will vary and should be determined empirically.

Selected P/N: 926-32202

Price

Quantity

Add to Cart

+ Add to WishlistAdded. Go to Wishlist

Generate an Autoquote

Resources

chat icon disabledLooking to chat with support? First, you will need to accept cookies.
Open Cookie Preferences
chat iconChat With Us
chat icon disabled