IRDye® 800CW Rabbit anti-HRP

Convert to NIR Fluorescence Detection with IRDye 800CW Rabbit anti-HRP

Immunogen

Peroxidase from horseradish roots

Purity and Specificity

The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with peroxidase from horseradish roots. It may cross-react with peroxidase from other sources. The conjugate has been specifically tested and qualified for Western blot applications

Application

  • Western Blot

Formulation

IRDye 800CW secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application Recommended Suggested Range
Odyssey® Western blot detection 1:15,000 1:5,000 - 1:25,000
Other User optimized

Optimum dilutions will vary and should be determined empirically.

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