IRDye® 680LT Donkey anti-Goat IgG Secondary Antibody

IRDye 680LT Donkey anti-Goat IgG Secondary Antibody

Immunogen

Goat IgG

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using goat IgG covalently linked to agarose. Based on ELISA, this antibody reacts with the heavy and light chains of goat IgG and sheep IgG. This antibody has been tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with human, mouse, rabbit, rat, chicken. guinea pig, hamster, swine, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

Applications

Recommended for:

  • Western Blot
  • Protein Array
  • Immunohistochemistry
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging

Not Recommended for:

  • Small Animal Imaging
  • In-Cell Western Assay
  • On-Cell Western Assay
Note: Do not use this secondary antibody in combination with any secondary antibody whose host is goat (for example, IRDye 800CW Goat anti-Rabbit) for two-color Western blot detection.

Formulation

IRDye 680LT secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application Recommended Suggested Range
Odyssey Western blot detection 1:20,000 1:20,000 - 1:50,000
Other User optimized

Optimum dilutions will vary and should be determined empirically.

Example Data

IRDye® 680LT Donkey anti-Goat IgG Secondary Antibody data
Western blot detection of actin in C32 lysate. C32 lysate (Santa Cruz P/N sc- 2205) was resolved on a 10% Bis-Tris gel and transferred to Odyssey® nitrocellulose (P/N 926-31090). The membrane was probed with goat anti-actin (Santa Cruz P/N sc-1616) followed by detection with IRDye 680LT Donkey anti-Goat IgG (P/N 926-68024).

Selected P/N: 926-68024,925-68024

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IRDye 680LT secondary antibodies are not intended for use in In-Cell Western assays or in vivo imaging applications.

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