IRDye® 680LT Protein Labeling Kits

IRDye 680LT Labeling Kits for Labeling Antibodies and Proteins.

IRDye 680LT Protein Labeling Kits can be used to label antibodies and other proteins for applications such as Western blots and microscopy.

High Molecular Weight

Optimized for labeling 1.0 mg of protein with molecular weight 45 - 200 kDa.

This kit contains:

  • 3 x 0.1 mg IRDye 680LT Reactive Dye vials
  • 0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9
  • 25 mL 1X PBS
  • 0.5 mL ultra-pure water
  • 3 x Pierce® Zeba Desalting Spin Columns (P/N 89891)
  • Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.
  • Pierce Zeba Desalting Spin column instructions
  • Protocol for IRDye 680LT Protein Labeling Kit - High MW

Low Molecular Weight

Optimized for labeling 1.0 mg of protein with molecular weight 14 - 45 kDa.

This kit contains:

  • 3 x 0.1 mg IRDye 680LT Reactive Dye vials
  • 0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9
  • 25 mL 1X PBS
  • 0.5 mL ultra-pure water
  • 3 x Pierce Zeba Desalting Spin Columns (P/N 89891)
  • Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.
  • Pierce Zeba Desalting Spin column instructions
  • Protocol for IRDye 680LT Protein Labeling Kit - Low MW

Microscale

Optimized for labeling 100 μg of protein with molecular weight 14 - 200 kDa.

This kit contains:

  • 3 x 0.1 mg IRDye 680LT Reactive Dye vials
  • 0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9
  • 25 mL 1X PBS
  • 0.5 mL ultra-pure water
  • 3 x Pierce Zeba Desalting Spin Columns (P/N 89892)
  • Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.
  • Pierce Zeba Desalting Spin Column instructions
  • Protocol for IRDye 680LT Protein Labeling Kit - Microscale

Selected P/N: 928-38066,928-38068,928-38070

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Note: IRDye 680LT dyes and labeling kits are not recommended for labeling small animal imaging agents or for performing In-Cell Western assays.

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