Label Primary and Secondary Amines with IRDye 680LT Infrared Dye.

IRDye® 680LT NHS Ester

Label Primary and Secondary Amines with IRDye 680LT Infrared Dye.

IRDye infrared dyes from LI-COR Biosciences are available for researchers developing applications using near-infrared (NIR) fluorescence.

Standard NHS ester chemistry is used to produce custom probes labeled with LI-COR IRDye infrared dyes. The NHS ester reactive group provides the functionality for labeling primary and secondary amines, such as lysine residues in proteins.

Which 700 Channel Dye Should I Use?

Note: IRDye 680LT dye products should not be used for small animal in vivo imaging or In‑Cell Western Assays. The higher level of fluorescent intensity creates high background making it unfavorable for use in these applications. We recommend using IRDye 680RD dye products for these applications.

IRDye® 800CW NHS Ester reaction
NHS Ester reacts with a primary aliphatic amine, such as lysine.
IRDye® 800CW NHS Ester amide bond
Dye label is attached through amide bond.

Dye Structure

IRDye® 680LT NHS Ester Structure


  • Chemical Formula: C54H52N3Na3O22S6
  • Molecular Weight: 1402.32 g/mol
  • Exact Mass: 1401.09

Absorption and Emission Spectra

Solvent Ext. Coeff. (M-1cm-1) Absorption Maxima Emission Maxima
Methanol 250,000 680 nm 694 nm
1X PBS 250,000 676 nm 693 nm

Absorption and Emission Spectra in 1X PBS

IRDye® 680LT Absorption and Emission Spectra

Selected P/N: 929-71010,929-71011,929-71500



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