The β-actin primary antibody can be used as an internal loading control (ILC) for normalization.
The expression of β-actin, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.
Once validated, β-actin primary antibodies can be used for the detection of β-actin when performing two-color detection.
Detect β-Actin Rabbit Primary Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.
Reactivity and Specificity
β-Actin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.
Do not aliquot the antibody.
|Properties||β-Actin Rabbit Monoclonal Antibody (P/N 926-42210)|
|Species Cross-Reactivity||Human, mouse, rat, monkey|
|Target Molecular Weight||45 kDa|
|Specificity/Sensitivity||May cross-react with γ-actin (cytoplasmic isoform)|
|Immunogen||A synthetic peptide (KLH-coupled) that corresponds to the residues near the amino-terminus of human β-actin|
|Tested Applications||Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F)|