Actin is a commonly-occurring structural housekeeping protein and has been used for Western blot normalization for many years.
You need to validate the expression of actin, or any housekeeping protein (HKP), to ensure that its expression does not change under experimental conditions. Otherwise, quantitation cannot accurately be performed.
Once validated, β-actin primary antibodies can be used for detection of β-actin as a normalization antibody when performing two-color detection.
Detect β-Actin Rabbit Primary Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.
Reactivity and Specificity
β-Actin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.
Do not aliquot the antibody.
|Properties||β-Actin Rabbit Monoclonal Antibody (P/N 926-42210)|
|Species Cross-Reactivity||Human, mouse, rat, monkey|
|Target Molecular Weight||45 kDa|
|Specificity/Sensitivity||May cross-react with γ-actin (cytoplasmic isoform)|
|Immunogen||A synthetic peptide (KLH-coupled) that corresponds to the residues near the amino-terminus of human β-actin|
|Tested Application||Western blot|