β-Actin Rabbit Monoclonal Antibody for Normalization

beta-Actin Rabbit Monoclonal Antibody

Actin is a commonly-occurring structural housekeeping protein and has been used for Western blot normalization for many years.

You need to validate the expression of actin, or any housekeeping protein (HKP), to ensure that its expression does not change under experimental conditions. Otherwise, quantitation cannot accurately be performed.

Once validated, β-actin primary antibodies can be used for detection of β-actin as a normalization antibody when performing two-color detection.

Detect β-Actin Rabbit Primary Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.

Other options for housekeeping protein normalization

Reactivity and Specificity

β-Actin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.

Do not aliquot the antibody.

Properties β-Actin Rabbit Monoclonal Antibody (P/N 926-42210)
Species Cross-Reactivity Human, mouse, rat, monkey
Target Molecular Weight 45 kDa
Isotype Rabbit IgG
Specificity/Sensitivity May cross-react with γ-actin (cytoplasmic isoform)

Selected P/N: 926-42210

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When using an HKP as your normalization strategy, it’s important to validate the HKP for each experiment to ensure its expression is stable.

Many factors can influence expression including tissue, treatment, and cell density.

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