IRDye 800CW NHS Ester will Label Primary and Secondary Amines.

IRDye® 800CW NHS Ester

IRDye 800CW NHS Ester will Label Primary and Secondary Amines.

IRDye infrared dyes from LI-COR Biosciences are available for researchers developing applications using near-infrared (NIR) fluorescence. These unique dyes are used in a variety of NIR imaging applications including Western blotting, plate-based assays, protein arrays, tissue section imaging, and molecular activity measurements.

Standard NHS ester chemistry is used to produce custom probes labeled with LI-COR IRDye infrared dyes. The NHS ester reactive group provides the functionality for labeling primary and secondary amines, such as lysine residues in proteins.

IRDye® 800CW NHS Ester reaction
NHS Ester reacts with a primary aliphatic amine, such as lysine.
IRDye® 800CW NHS Ester amide bond
Dye label is attached through amide bond.

Dye Structure

IRDye® 800CW NHS Ester Structure


  • Chemical Formula: C50H54N3Na3O17S4
  • Molecular Weight: 1166.20 g/mol
  • Exact Mass: 1165.20

Absorption and Emission Spectra

Solvent Ext. Coeff. (M-1cm-1) Absorption Maxima Emission Maxima
Methanol 300,000 778 nm 794 nm
Water 240,000 774 nm 789 nm
1X PBS 240,000 774 nm 789 nm
PBS: Methanol 270,000 777 nm 791 nm

Absorption and Emission Spectra in 1X PBS

IRDye 800CW Absorption and Emission Spectra

Selected P/N: 929-70020,929-70021,929-70022,929-70023,929-70024,929-70025,929-70026



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