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MPX™ Blotting System for Multiple-Target Western Blots

MPX Blotting System to Optimize Multiplex Western Detection.

The MPX Blotting System is ideal for any multiple-target Western blot application. This system increases Western blot throughput, maximizes efficiency, and reduces relative overall cost.

Screen Samples or Optimize Blotting Conditions with the MPX Blotting System

Screen multiple samples and multiple targets all on the same blot without cutting, stripping, or cross-contamination.

Use the MPX Blotter for a variety of assays, such as primary and secondary antibody screening, monoclonal antibody screening, blocking buffer optimization as well as multi-target probing of 1 - 4 samples on a single membrane. Most common Western procedures that generate a PVDF or nitrocellulose blot of 7 cm x 8.5 cm can be adapted to the MPX format.

Simple, Convenient Workflow

MPX Blotting System workflow

Channel ports are conveniently spaced, staggered, and beveled to create a simple workflow for using both standard and multi-channel pipettes. The low volume channel ports accommodate a maximum volume of 160 µL, thus conserving precious and costly antibody. The nesting dovetail joint design allows multiple MPX units to be linked together for easy-to-handle multiple-blot processing.

Screen Up To 48 Targets on One Blot

Processing is as simple as clamping the blot into the MPX, creating up to 24 independent channels. The range of usable channels per sample is relative to comb-size – from 24 on a single sample to four on a four-sample blot.

Using LI-COR’s selection of IRDye® dye-conjugated secondary antibodies on the Odyssey Imagers provides a maximum of 48-targets on a single membrane— two per channel—and the option for quantitative analysis. Other detection methods, such as standard chemiluminescence and colorimetric, can provide a maximum 24 targets.

MPX Blotting System Specifications

  • Overall Size: 15.5 cm x 8.5 cm x 3.5 cm (L x W x H)
  • Membrane Size: 7 cm x 8.5 cm (H x L)
  • Channel Dimensions: 50 mm x 1.5 mm x 1.5 mm (L x W x H)
  • Number of Channels: 24
  • Total Volume per Channel Including Port: 160 µL

MPX Western Blot with One Sample and Multiple Targets

MPX Blotting System example data
Representative MPX Western Blot with one sample and multiple targets.

Selected P/N: 921-00000

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