IRDye 680RD Carboxylate is Non-Reactive and an Ideal Control.

IRDye® 680RD Carboxylate

IRDye 680RD Carboxylate is Non-Reactive and an Ideal Control.

Assays that use IRDye 680RD conjugates (such as small animal imaging and cell binding assays) may require a "dye-only" control for potential effects or retention of the dye.

Carboxylate (non-reactive) form of IRDye 680RD is an ideal control.

Note: The carboxylate dye has no reactive group and cannot be used for labeling.

How is IRDye 680RD Carboxylate Used?

In vitro Cell-based Assays

As a control for cell-based assays that monitor binding of a dye-labeled agent.

  • Validation of optical agents for in vivo administration
  • Evaluation of binding specificity

In vivo Imaging

For evaluation of behavior and clearance of the dye itself.

  • Timing of dye clearance from the animal's body
  • Retention of dye in certain organs or sites (e.g., liver or kidneys)

Labeling Reaction Reference

As a standard to determine the amount of unreacted ("free") dye after IRDye 680RD conjugation and purification.

Residual unreacted dye may cause:

  • Artificially high values when dye/protein (D/P) ratio is calculated
  • Increased background fluorescence in biological assays

Dye Structure

IRDye 680RD Carboxylate Structure


  • Chemical Formula: C39H41CIN3Na3O11S3
  • Molecular Weight: 928.37 g/mol
  • Exact Mass: 927.13

Selected P/N: 929-08980



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