β-Tubulin primary antibodies can be used for detection of β-tubulin by Western blot or by In-Cell Western™ assay.
You need to validate the expression of tubulin, or any housekeeping protein (HKP), to ensure that its expression does not change under experimental conditions. Otherwise, quantitation cannot accurately be performed.
Once validated, β-tubulin primary antibodies can be used for detection of β-tubulin as a normalization antibody when performing two-color detection.
Detect β-Tubulin Rabbit Polyclonal Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.
Reactivity and Specificity
β-Tubulin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.
Do not aliquot the antibody.
|Properties||β-Tubulin Rabbit Polyclonal Antibody (P/N 926-42211)|
|Species Cross-Reactivity||Human, mouse, rat, monkey, bovine|
|Target Molecular Weight||55 kDa|
|Specificity/Sensitivity||Detects endogenous levels of total β-tubulin protein. Does not cross-react with recombinant α-tubulin.|
|Immunogen||A synthetic peptide (KLH-coupled) that corresponds to the sequence of total β-tubulin, and does not cross-react with recombinant α-tubulin|
|Tested Application||Western blot|