The β-Tubulin primary antibody can be used as an internal loading control (ILC) for normalization.
The expression of β-tubulin, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.
Once validated, β-tubulin primary antibodies can be used for the detection of β-tubulin when performing two-color detection.
Detect β-Tubulin Rabbit Polyclonal Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.
Reactivity and Specificity
β-Tubulin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.
Do not aliquot the antibody.
|Properties||β-Tubulin Rabbit Polyclonal Antibody (P/N 926-42211)|
|Species Cross-Reactivity||Human, mouse, rat, monkey, bovine|
|Target Molecular Weight||55 kDa|
|Specificity/Sensitivity||Detects endogenous levels of total β-tubulin protein. Does not cross-react with recombinant α-tubulin.|
|Immunogen||A synthetic peptide (KLH-coupled) that corresponds to the sequence of total β-tubulin, and does not cross-react with recombinant α-tubulin|
|Tested Applications||Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F)|