Intercept™ (PBS) Blocking Buffer

Try Intercept (PBS) Blocking Buffer for Outstanding Performance

Intercept (PBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for reproducible quantitative Western blots and other immunoassays. Intercept (PBS) Blocking Buffer is the blocking buffer of choice for near-infrared fluorescent applications when using a phosphate-buffered saline (PBS) system.

  • Contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies.
  • Excellent choice for biotin-streptavidin detection.
  • Suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays.

For larger pack sizes, please contact your local sales office.

Formulation

Intercept (PBS) Blocking Buffer is a ready-to-use blocker formulation in PBS. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection. It is stored at 4 °C and is also available in kits with Immobilon®-FL PVDF and nitrocellulose membranes.

Figure 1: Western blot protocol demonstrating the comparison of Intercept Blocking Buffer to Odyssey Blocking Buffer. Blots with serial dilution of mouse heart tissue extract were probed for Stat3 Mouse mAb and p38 MAPK Rabbit Ab, detected with IRDye® 800CW Goat anti-Mouse IgG (P/N 925-32210) and IRDye 680LT Goat anti-Rabbit IgG (P/N 925-68021), and scanned together on an Odyssey CLx Imaging System.

Intercept (PBS) Blocking Buffer can be used for many immunoassays and applications, including:

Selected P/N: 927-70001,927-70003,927-70010

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