IRDye® 800CW Donkey anti-Chicken Secondary Antibody

IRDye 800CW Donkey anti-Chicken Secondary Antibody

Immunogen

Chicken IgY, whole molecule. IgY is the original designation for the IgG-like protein found in both serum and egg yolk.

Purity and Specificity

The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis and/or ELISA, this antibody reacts with whole molecule chicken IgY, and with the light chains of other chicken immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, goat, guinea pig, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

Applications

Highly recommended for:

  • Western Blot
  • In-Cell Western Assay
  • On-Cell Western Assay
  • Protein Array
  • Immunohistochemistry
  • Small Animal Imaging
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging
  • Virus Titration Assay

Formulation

IRDye 800CW secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application Recommended Suggested Range
Odyssey Western blot detection 1:15,000 1:5,000 - 1:25,000
Other User optimized

Optimum dilutions will vary and should be determined empirically.

Example Data

IRDye® 800CW Donkey anti-Chicken Secondary Antibody data
Detection of actin with IRDye 800CW Donkey anti-chicken. Odyssey® One-Color Molecular Weight Marker (P/N 928-40000) and C32 lysate, 3 µg – 0.01 µg (Santa Cruz P/N sc-2205) were resolved on a 10% Bis Tris gel and transferred to Odyssey Nitrocellulose membrane (P/N 926-31092). Membrane was blocked with Odyssey Blocking Buffer (P/N 927-40000) followed by detection with chicken anti-actin (GenWay P/N 15-288-23014) and IRDye 800CW Donkey anti-Chicken (P/N 926-32218).

Selected P/N: 926-32218,925-32218

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