IRDye® 800CW Streptavidin

IRDye 800CW Streptavidin for Biotin Detection

IRDye 800CW Streptavidin is supplied as a liquid in buffer containing 10 mM phosphate, 183 mM NaCl, 2.7 nM KCl, pH 7.4 with sodium azide 0.005% (w/v) as a preservative.

To use, centrifuge briefly before use to eliminate aggregates that may have formed in solution. This will reduce non-specific background staining. A final concentration of 0.2 to 1.0 µg/ml (1:1,000 to 1:5,000) is usually satisfactory for most applications; however, appropriate dilution may need to be determined empirically.

For membrane-based applications and In-Gel Westerns, it is recommended to add SDS (0.02% to 0.1% final concentration), in addition to Tween® 20 (0.1 to 0.2% final concentration) during the detection incubation step to reduce non-specific background staining.

Recommended Dilutions

Application Suggested Range Tween 20* SDS*
Odyssey® Western blot detection 1:1,000 - 1:5,000 0.1 - 0.2% (v/v) 0.02 - 0.1% (v/v)
Other User optimized User optimized User optimized

Optimum dilutions will vary and should be determined empirically.

* Added to reduce non-specific background staining.

See the complete line of IRDye Streptavidins.

Selected P/N: 926-32230,925-32230

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