Rat IgG, whole molecule
Purity and Specificity
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis and/or ELISA, this antibody reacts with whole molecule rat IgG, and with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse, bovine, horse, human, and rabbit serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.
Highly recommended for:
- Western Blot
- In-Cell Western™ Assay
- On-Cell Western Assay
- Protein Array
- Small Animal Imaging
- 2D Gel Detection
- Tissue Section Imaging
- Virus Titration Assay
IRDye 800CW Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
|Odyssey Western blot detection||1:20,000||1:5,000 - 1:25,000|
|In-Cell Western Assay detection||1:800||1:200 - 1:1,200|
Optimum dilutions will vary and should be determined empirically.
- P/N 925-32219: RRID AB_2721932
- P/N 926-32219: RRID AB_1850025