IRDye® 800CW Goat anti-Rat IgG Secondary Antibody

IRDye 800CW Goat anti-Rat IgG Secondary Antibody

Immunogen

Rat IgG, whole molecule

Purity and Specificity

The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis and/or ELISA, this antibody reacts with whole molecule rat IgG, and with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse, bovine, horse, human, and rabbit serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

Applications

Highly recommended for:

  • Western Blot
  • In-Cell Western Assay
  • On-Cell Western Assay
  • Protein Array
  • Immunohistochemistry
  • Small Animal Imaging
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging
  • Virus Titration Assay

Formulation

IRDye 800CW secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application Recommended Suggested Range
Odyssey Western blot detection 1:15,000 1:5,000 - 1:25,000
Other User optimized

Optimum dilutions will vary and should be determined empirically.

Example Data

IRDye® 800CW Goat anti-Rat IgG Secondary Antibody data
Western blot detection of tubulin in C32 lysate. C32 lysate (Santa Cruz P/N sc- 2205) was resolved on a 10% Tris-Glycine gel and transferred to Odyssey® nitrocellulose (P/N 926-31090). The membrane was probed with rat anti-tubulin (Novus Biologicals P/N NB600-506) followed by detection with IRDye 800CW Goat anti-Rat IgG (P/N 926-32219).

Selected P/N: 926-32219,925-32219

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