GAPDH Rabbit Monoclonal Antibody for Normalization

GAPDH Rabbit Monoclonal Antibody for Normalization

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a constitutively expressed housekeeping protein (HKP). The GAPDH primary antibody can be used as an internal loading control for normalization.

The expression of GAPDH, or any HKP, should be validated to ensure that its expression does not change under experimental conditions.

Once validated, GAPDH primary antibodies can be used for the detection of GAPDH when performing multiplex Western blot detection.

Detect GAPDH Rabbit Monoclonal Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.

Other options for housekeeping protein normalization

Reactivity and Specificity

GAPDH antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.

Do not aliquot the antibody.

Properties GAPDH Rabbit Monoclonal Antibody (P/N 926-42216)
Species Cross-Reactivity Human, mouse, rat, monkey
Target Molecular Weight 37 kDa
Isotype Rabbit IgG
Specificity/Sensitivity Detects endogenous levels of total GAPDH protein. May cross-react with pig
Immunogen A synthetic peptide that corresponds to residues near the carboxy terminus of human GAPDH
Tested Applications Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

GAPDH Rabbit Monoclonal Antibody in HeLa, NIH/3T3, and COS-7 Lysates

Beta tubuin detected in various cell lines
GAPDH Rabbit Monoclonal Antibody detected in HeLa, NIH/3T3, and COS-7 lysates. Lysates were diluted from 2.5 μg to 156 ng. Lysates were separated on 4-12% Bis-Tris gels, electrophoresed at 200V for 45 minutes in MES Running Buffer, and transferred to nitrocellulose membranes in Tris-Glycine buffer at 100V for 65 minutes. Blots were blocked with Intercept® (PBS) Protein-Free Blocking Buffer (P/N 927-90001), probed with GAPDH Rabbit Monoclonal Antibody, and detected on an Odyssey® CLx Imager.

Selected P/N: 926-42216

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When using an HKP as your normalization strategy, it’s important to validate the HKP for each experiment to ensure its expression is stable.

Many factors can influence expression including tissue, treatment, and cell density.

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