Cytochrome c oxidase is localized to the inner mitochondrial membrane. The COX IV antibody can be used as a mitochondrial loading control and a loading control for normalizing low-expressing target proteins.
You need to validate the expression of COX IV, or any housekeeping protein (HKP), to ensure that its expression does not change under experimental conditions. Otherwise, quantitation cannot accurately be performed.
Once validated, COX IV primary antibodies can be used for detection of COX IV as a normalization antibody when performing two-color detection.
COX IV Rabbit Monoclonal Primary Antibody can be used for detection of COX IV by Western blot, or as a normalization antibody when performing two-color detection. Detect this primary antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.
Reactivity and Specificity
COX IV antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.
Do not aliquot the antibody.
|Properties||COX IV Rabbit Monoclonal Antibody (P/N 926-42214)|
|Species Cross-Reactivity||Human, rabbit, monkey, zebrafish, bovine, pig|
|Target Molecular Weight||17 kDa|
|Specificity/Sensitivity||Detects endogenous levels of total COX IV protein.|
|Immunogen||A synthetic peptide that corresponds to the residues surrounding Lys29 of human COX IV|
|Tested Application||Western blot|