COX IV Rabbit Primary Antibody for Normalization

COX IV Antibody is Best for Low-Expressing Protein Normalization.

Cytochrome c oxidase is localized to the inner mitochondrial membrane. The COX IV antibody can be used as a mitochondrial loading control and a loading control for normalizing low-expressing target proteins.

You need to validate the expression of COX IV, or any housekeeping protein (HKP), to ensure that its expression does not change under experimental conditions. Otherwise, quantitation cannot accurately be performed.

Once validated, COX IV primary antibodies can be used for detection of COX IV as a normalization antibody when performing two-color detection.

COX IV Rabbit Monoclonal Primary Antibody can be used for detection of COX IV by Western blot, or as a normalization antibody when performing two-color detection. Detect this primary antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.

Other options for housekeeping protein normalization

Reactivity and Specificity

COX IV antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.

Do not aliquot the antibody.

Properties COX IV Rabbit Monoclonal Antibody (P/N 926-42214)
Species Cross-Reactivity Human, rabbit, monkey, zebrafish, bovine, pig
Target Molecular Weight 17 kDa
Isotype Rabbit IgG
Specificity/Sensitivity Detects endogenous levels of total COX IV protein.
Immunogen A synthetic peptide that corresponds to the residues surrounding Lys29 of human COX IV
Tested Application Western blot

COX IV Antibody Detected in Various Cell Lines

COX IV antibody detected in various cell lines
COX IV was detected in Jurkat, HeLa, and COS-7 cell lines.

Selected P/N: 926-42214

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When using an HKP as your normalization strategy, it’s important to validate the HKP for each experiment to ensure its expression is stable.

Many factors can influence expression including tissue, treatment, and cell density.

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