Cytochrome c oxidase is localized to the inner mitochondrial membrane. The COX IV antibody can be used as a mitochondrial loading control and an internal loading control (ILC) and is particularly effective when normalizing low-expressing target proteins.
The expression of COX IV, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.
Once validated, COX IV primary antibodies can be used for the detection of COX IV when performing two-color detection.
Detect COX IV Rabbit Monoclonal Primary Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.
Reactivity and Specificity
COX IV antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.
Do not aliquot the antibody.
|Properties||COX IV Rabbit Monoclonal Antibody (P/N 926-42214)|
|Species Cross-Reactivity||Human, rabbit, monkey, zebrafish, bovine, pig|
|Target Molecular Weight||17 kDa|
|Specificity/Sensitivity||Detects endogenous levels of total COX IV protein.|
|Immunogen||A synthetic peptide that corresponds to the residues surrounding Lys29 of human COX IV|
|Tested Applications||Western blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F)|