COX IV Rabbit Primary Antibody for Normalization

COX IV Antibody is Best for Low-Expressing Protein Normalization.

Cytochrome c oxidase is localized to the inner mitochondrial membrane. The COX IV antibody can be used as a mitochondrial loading control and an internal loading control (ILC) and is particularly effective when normalizing low-expressing target proteins.

The expression of COX IV, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.

Once validated, COX IV primary antibodies can be used for the detection of COX IV when performing two-color detection.

Detect COX IV Rabbit Monoclonal Primary Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.

Other options for housekeeping protein normalization

Reactivity and Specificity

COX IV antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.

Do not aliquot the antibody.

Properties COX IV Rabbit Monoclonal Antibody (P/N 926-42214)
Species Cross-Reactivity Human, rabbit, monkey, zebrafish, bovine, pig
Target Molecular Weight 17 kDa
Isotype Rabbit IgG
Specificity/Sensitivity Detects endogenous levels of total COX IV protein.
Immunogen A synthetic peptide that corresponds to the residues surrounding Lys29 of human COX IV
Tested Applications Western blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F)

COX IV Antibody Detected in Various Cell Lines

COX IV antibody detected in various cell lines
COX IV was detected in Jurkat, HeLa, and COS-7 cell lines.

Selected P/N: 926-42214

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When using an HKP as an ILC for normalization, it’s important to validate the HKP for each experiment to ensure its expression is stable.

Many factors can influence expression including tissue, treatment, and cell density.

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