CellTag 520 Stain for In-Cell Western Assays

CellTag 520 Stain for Accurate Normalization in ICW Assays.

CellTag 520 Stain is a visible fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications.

The stain accumulates in both the nucleus and cytoplasm of permeabilized cells, and provides linear fluorescent signal across a wide range of cell types and cell numbers.

CellTag 520 Stain is detected in the 520 nm channel. CellTag 520 Stain is applied to the cells during incubation with an IRDye® Secondary Antibodies (e.g., IRDye 680RD or IRDye 800CW), and enables accurate measurement of target protein levels with much higher throughput than Western blotting.

Properties

  • Size: 2 x 50 nmole (lyophilized)
  • λExEm: 548/563 nm
  • Recommended concentration: 0.1 μM

Linear Relationship Between Fluorescence and Cell Number

Two-fold serial dilutions of NIH3T3 cells were plated in a 96-well plate. Cells were fixed, permeabilized, stained with CellTag 520 Stain (0.1 μM), and detected with the Odyssey M Imaging System.

Resources

chat iconChat With Us
chat icon disabled