The In-Cell Western Assay is an immunocytochemical assay that uses fluorescence to detect and quantify proteins in cells that have been cultured in microplates. Detecting proteins in their cellular context increases quantification precision.
This process allows for higher throughput compared to Western blotting and eliminates typical Western blotting steps such as cell lysate preparation, electrophoresis, and membrane transfer. Cost per well is reduced to a fraction of the cost of typical screening methods with CellTag™ Stain In-Cell Western Kits.
CellTag Stains are a fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western applications. The stain accumulates in both the nucleus and cytoplasm of permeabilized cells and provides linear fluorescent signal across a wide range of cell types and cell numbers. CellTag Stains are applied to the cells during incubation with IRDye® Secondary Antibodies and enable accurate measurement of target protein levels with much higher throughput than Western blotting.
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| Product Name | Price |
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CellTag™ 520 Stain for In-Cell Western™ Assays
CellTag 520 Stain is a visible fluorescent, non-specific cell stain that provides linear fluorescent signal across a wide range of cell types and cell numbers for accurate normalization in several multiwell plate assays. |
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CellTag™ 700 Stain for In-Cell Western™ Assays
CellTag 700 Stain is a near-infrared fluorescent, non-specific cell stain that provides linear fluorescent signal across a wide range of cell types and cell numbers for accurate normalization in several multiwell plate assays. |
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GAM CellTag™ 700 Stain ICW Kit I
This kit includes detection reagents, IRDye 800CW Goat anti-Mouse Secondary Antibody and Intercept (TBS) Blocking Buffer, as well as CellTag 700 Stain for normalization. |
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GAM/GAR CellTag™ 520 Stain ICW Kit III
This kit includes detection reagents, IRDye 800CW Goat anti-Mouse Secondary Antibody, IRDye 680RD Goat anti-Rabbit Secondary Antibody, and Intercept (TBS) Blocking Buffer, as well as CellTag 520 Stain for normalization. |
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GAR CellTag™ 700 Stain ICW Kit II
This kit includes detection reagents, IRDye 800CW Goat anti-Rabbit Secondary Antibody and Intercept (TBS) Blocking Buffer, as well as CellTag 700 Stain for normalization. |
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GAR/GAM CellTag™ 520 Stain ICW Kit IV
This kit includes detection reagents, IRDye 800CW Goat anti-Rabbit Secondary Antibody, IRDye 680RD Goat anti-Mouse Secondary Antibody, and Intercept (TBS) Blocking Buffer, as well as CellTag 520 Stain for normalization. |
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Sapphire700™ Stain for Cell Viability Assays and In-Cell Western™ Assay Normalization
Sapphire700 Stain is a non-specific cell stain that accumulates in both nucleus and cytoplasm of fixed cells or dead cells. |