Guinea Pig IgG, whole molecule
Purity and Specificity
Isolation of specific antibodies was accomplished by affinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis and/or ELISA, this antibody reacts with whole molecule guinea pig IgG as well as the light chains common to other guinea pig immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, chicken, goat, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications.
Highly recommended for:
- Western Blot
- In-Cell Western Assay
- On-Cell Western Assay
- Protein Array
- Small Animal Imaging
- 2D Gel Detection
- Tissue Section Imaging
- Virus Titration Assay
IRDye 800CW secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
|Odyssey Western blot detection||1:15,000||1:5,000 - 1:25,000|
|In-Cell Western assay detection||1:800||1:200 - 1:1,200|
Optimum dilutions will vary and should be determined empirically.