IRDye® 680LT Goat anti-Mouse IgG2b-Specific Secondary Antibody

IRDye 680LT Goat anti-Mouse IgG2b Secondary Antibody.

Immunogen

Mouse IgG2b paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography on mouse IgG2b covalently linked to agarose. Based on ELISA and flow cytometry, the antibody reacts with the heavy chain of mouse IgG2b. This antibody was tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse IgM, IgG1, IgG2a, IgG3, and IgA, pooled human sera, and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot applications. Results with your primary antibody may vary and specificity should be confirmed prior to performing two-color detection.

Applications

Recommended for:

  • Western Blot
  • Protein Array
  • Immunohistochemistry
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging

Not Recommended for:

  • Small Animal Imaging
  • In-Cell Western Assay
  • On-Cell Western Assay

Formulation

IRDye 680LT secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application Recommended Suggested Range
Odyssey Western blot detection 1:20,000 1:20,000 - 1:50,000
Other User optimized

Optimum dilutions will vary and should be determined empirically.

Example Data

IRDye 680LT Goat anti-Mouse IgG2b-Specific Secondary Antibody data
Western blot detection of p53 in various lysates. Lanes 1-5: Odyssey® One-Color Molecular Weight Marker (P/N 928-40000), Hela, COS7, A431, and C32 cell lysates. The membrane was blocked with Odyssey Blocking Buffer (P/N 927-40000) and probed with mouse anti-p53 (IgG2a) (Santa Cruz P/N sc-126) and Mouse anti-β Actin (IgG2b) (P/N 926-42212) followed by detection with IRDye 800CW Goat anti-Mouse IgG2a (P/N 926-32351) and IRDye 680LT Goat anti-Mouse IgG2b (P/N 926-68052).

Selected P/N: 926-68052

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IRDye 680LT secondary antibodies are not intended for use in In-Cell Western assays or in vivo imaging applications.

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