CellTag 700 Stain is a near-infrared fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications.
The stain accumulates in both the nucleus and cytoplasm of permeabilized cells, and provides linear fluorescent signal across a wide range of cell types and cell numbers.
CellTag 700 Stain is detected in the 700 nm channel of Odyssey® CLx, Classic, and Sa Imaging Systems. CellTag 700 Stain is applied to the cells during incubation with an IRDye® 800CW secondary antibody, and enables accurate measurement of target protein levels with much higher throughput than Western blotting.
Properties
- Size: 2 x 10 nmole (lyophilized)
- λEx/λEm: 675/697 nm
- Recommended concentration: 0.2 μM
Linear Relationship Between Fluorescence and Cell Number
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