CellTag 700 Stain for In-Cell Western Assays

CellTag 700 Stain for Accurate Normalization in ICW Assays.

CellTag 700 Stain is a near-infrared fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications.

The stain accumulates in both the nucleus and cytoplasm of permeabilized cells, and provides linear fluorescent signal across a wide range of cell types and cell numbers.

CellTag 700 Stain is detected in the 700 nm channel of Odyssey® CLx, Classic, and Sa Imaging Systems. CellTag 700 Stain is applied to the cells during incubation with an IRDye® 800CW secondary antibody, and enables accurate measurement of target protein levels with much higher throughput than Western blotting.

Properties

  • Size: 2 x 10 nmole (lyophilized)
  • λExEm: 675/697 nm
  • Recommended concentration: 0.2 μM

Linear Relationship Between Fluorescence and Cell Number

Two-fold serial dilutions of A431 and NIH/3T3 cells were plated in 96-well plates. Cells were fixed, permeabilized, stained with CellTag 700 Stain (0.2 μM), and detected with the Odyssey Classic Infrared Imaging System. Trim Signal values were used to generate the graphs.

Selected P/N: 926-41090

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